Chestnut blight caused by Cryphonectria parasitica is a severe disease worldwide affecting chestnut trees. One pathway for its long-range transmission is grafting by using non-symptomatic scions. The effects of five disinfectants, 80% ethanol, 2.5% NaOCl, 5% H₂O₂, 2% copper sulphate and Lysol® (Brand III), were evaulated on artificially inoculated scions of 15–20 cm with a spore suspension (1 × 10⁶ conidia/ml) of C. parasitica. Effects of these disinfectants on natural fungal flora and the viability of the scion woods were also investigated in vitro and in vivo conditions. Effectiveness of the disinfectants was evaluated by plating 10 buds taken from five random scions on PDA and counting the growing fungal colonies after 7 days of incubation at 22 °C, with a 12-h lighting period. A total of 40 buds, 10 from each repetition, were cultured for each treatment. The growth of C. parasitica and other fungi were investigated under a stereomicroscope. No growth of C. parasitica was observed on the treated scions while it grew on all of the contaminated control buds (100%). The growth of other fungi was the lowest for the Lysol® treated scions. The effect of the disinfectant treatment on the viability of the scion woods were tested by using 45 scion woods for each treatment and the maximum viability ratio was obtained in distilled water, followed by NaOCl, copper sulphate, ethanol, H₂O₂ and Lysol® treatment.

由寄生C（Cryphonectria parasitica）引起的栗疫病在世界范围内是严重的疾病，影响栗树。远距离传播的途径之一是使用无症状接穗。在带有孢子悬浮液的15-20 cm人工接种接穗上评估了五种消毒剂的效果：80％乙醇，2.5％NaOCl，5％H ₂ O _2，2％硫酸铜和Lysol®（品牌III）。 ×10 ⁶分生孢子/ ml）。还在体外和体内研究了这些消毒剂对天然真菌菌群和接穗木材活力的影响。条件。通过将五个随机接穗上的10个芽接种在PDA上，并在22°C孵育7天后光照12小时来计数生长的真菌菌落，来评估消毒剂的有效性。每种处理共培养40个芽，每个重复10个。在立体显微镜下研究了寄生寄生衣藻和其他真菌的生长。无寄生梭状芽胞杆菌的生长在所有受污染的对照芽（100％）上生长的处理后的接穗上均观察到了这种现象。Lysol®处理的接穗中其他真菌的生长最低。消毒处理对接穗木生存力的影响通过每次处理使用45个接穗木进行测试，并在蒸馏水中，然后是NaOCl，硫酸铜，乙醇，H ₂ O ₂和Lysol®中获得最大生存率。治疗。