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Effectiveness and efficacy of superficial disinfectants to prevent mechanical transmission of Cryphonectria parasitica from chestnut scion woods

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Abstract

Chestnut blight caused by Cryphonectria parasitica is a severe disease worldwide affecting chestnut trees. One pathway for its long-range transmission is grafting by using non-symptomatic scions. The effects of five disinfectants, 80% ethanol, 2.5% NaOCl, 5% H2O2, 2% copper sulphate and Lysol® (Brand III), were evaulated on artificially inoculated scions of 15–20 cm with a spore suspension (1 × 106 conidia/ml) of C. parasitica. Effects of these disinfectants on natural fungal flora and the viability of the scion woods were also investigated in vitro and in vivo conditions. Effectiveness of the disinfectants was evaluated by plating 10 buds taken from five random scions on PDA and counting the growing fungal colonies after 7 days of incubation at 22 °C, with a 12-h lighting period. A total of 40 buds, 10 from each repetition, were cultured for each treatment. The growth of C. parasitica and other fungi were investigated under a stereomicroscope. No growth of C. parasitica was observed on the treated scions while it grew on all of the contaminated control buds (100%). The growth of other fungi was the lowest for the Lysol® treated scions. The effect of the disinfectant treatment on the viability of the scion woods were tested by using 45 scion woods for each treatment and the maximum viability ratio was obtained in distilled water, followed by NaOCl, copper sulphate, ethanol, H2O2 and Lysol® treatment.

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The data that support the findings of this study are available from the corresponding author, [BA], upon reasonable request.

References

  • Amponsah, N. T., Walter, B. M., Fisher, M., Frijters, L., Hedderley, D. I., & Scheper, R. W. A. (2016). Hot water disinfection treatment to eliminate Neonectria ditissima latent infections in apple scion wood. New Zealand Plant Protection, 69, 221–229.

    Article  Google Scholar 

  • Anagnostakis, S. L. (1987). Chestnut blight: the classical problem of an introduced pathogen. Mycologia, 79(1), 23–37.

    Article  Google Scholar 

  • Anonymous. (2018). American chestnut cooperators, foundation restoration efforts grafting for blight resistance. https://www.accf-online.org/grafting.html. Accessed 2 June 2020.

  • Barreneche, T., Botta, R., & Robin, C. (2019). Advances in breeding of chestnuts. In U. Serdar & D. W. Fulbright (Eds.), Achieving sustainable cultivation of tree nuts (pp. 227–233). London: Burleigh Dodds.

    Google Scholar 

  • Ciordia, M., García, J. C., & Loureiro, M. D. (2020). Hot water treatment: an effective method for disinfecting Castanea sativa mill. dormant scions against Dryocosmus kuriphilus Yasumatsu. Pest Management Science, 76(5), 1944–1948.

    Article  CAS  Google Scholar 

  • Ćurković Perica, M., Krstin, L., Katanic, Z., Ježić, M., Nuskern, L., Poljak, I., et al. (2017). Hypovirulent Cryphonectria parasitica strains for biocontrol of chestnut blight in nurseries. IOBC-WPRS Bulletin, 124, 238–239.

    Google Scholar 

  • Fisher, B. M., Hedderley, D. I., Amponsah, N. T., & Scheper, R. W. A. (2018). Effect of heat or chemical disinfection on the viability of ‘Fuji’apple graft wood. New Zealand Plant Protection, 71, 354–354.

    Article  Google Scholar 

  • Ghorbel, A., Chatibi, A., Thaminy, S., & Kchouk, M. (1997). Micrografting of almond (Prunus dulcis (Miller) DA Webb) cv. Achak. In II International Symposium on Pistachios and Almonds 470, 1997 (pp. 429–433).

  • Gonthier, P., & Robin, C. (2019). Diseases. In G. Beccaro, A. Alma, G. Bounous, & J. Gomes-Laranjo (Eds.), The chestnut handbook: Crop & forest management (pp. 297–315). London: Taylor & Francis Group.

    Chapter  Google Scholar 

  • Hartmann, H. T., Kester, D. E., Davies, F. T., & Geneve, R. L. (2014). Hartmann and Kester’s Plant Propagation: Principles and Practices. London: Pearson Education.

  • Li, R., Baysal-Gurel, F., Abdo, Z., Miller, S. A., & Ling, K. (2015). Evaluation of disinfectants to prevent mechanical transmission of viruses and a viroid in greenhouse tomato production. Virology Journal, 12(1), 5.

    Article  CAS  Google Scholar 

  • Lione, G., Giordano, L., Turina, M., & Gonthier, P. (2020). Hail-Induced infections of the chestnut blight pathogen Cryphonectria parasitica depend on wound size and may lead to severe diebacks. Phytopathology, 110(7), 1280–1293.

    Article  Google Scholar 

  • Panagou, E. Z., Vekiari, S. A., Sourris, P., & Mallidis, C. (2005). Efficacy of hot water, hypochlorite, organic acids and natamycin in the control of post-harvest fungal infection of chestnuts. The Journal of Horticultural Science and Biotechnology, 80(1), 61–64.

    Article  CAS  Google Scholar 

  • Pasche, S., Crovadore, J., Pelleteret, P., Jermini, M., Mauch-Mani, B., Oszako, T., et al. (2016). Biological control of the latent pathogen Gnomoniopsis smithogylvyi in European chestnut grafting scions using Bacillus amyloliquefaciens and Trichoderma atroviride. Dendrobiology, 75, 113–122.

    Article  CAS  Google Scholar 

  • Rahemi, A. R., Taghavi, T., & Dale, A. (2016). A simple method to improve hazelnut grafting. Journal of Nuts, 7(2), 83–87.

    Google Scholar 

  • Rigling, D., & Prospero, S. (2018). Cryphonectria parasitica, the causal agent of chestnut blight: invasion history, population biology and disease control. Molecular Plant Pathology, 19(1), 7–20.

    Article  CAS  Google Scholar 

  • Roistacher, C., Navarro, L., & Murashige, T. (1976). Recovery of citrus selections free of several viruses, exocortis viroid, and Spiroplasma citri by shoot-tip grafting in vitro. In 7th International Organization of Citrus Virologists Conference Proceedings, (pp. 186–193).

  • Soylu, A. (2004). Kestane yetiştiriciliği ve özellikleri (Genişletilmiş II. Baskı). [Chestnut cultivation]. Turkey: HASAD Publication. (in Turkish).

    Google Scholar 

  • Tosun, F. (1991). Tarımda uygulamalı istatistik metotları [Agricultural statistics], Vol. 1. Samsun: Ondokuz Mayis University, Ziraat Fak. Ders Notu). (in Turkish).

  • TUİK. (2020). Chestnut growing statistics. https://www.tuik.gov.tr. Accessed 2 June 2020.

  • Turchetti, T. (1978). Some observations on the “hypovirulence” of chestnut blight in Italy. In Proceedings of the American Chestnut Symposium, West Virginia University Books, Morgantown, West Virginia, (pp. 92–94).

  • Turchetti, T. (1982). Hypovirulence in chestnut blight (Endothia parasitica [Murr.] And.) and some practical aspects in Italy. European Journal of Forest Pathology, 12(6), 414–416.

    Article  Google Scholar 

  • Turchetti, T., & Maresi, G. (1988). Mixed inoculum for the biological control of chestnut blight 1. EPPO Bulletin, 18(1), 67–72.

    Article  Google Scholar 

  • Warmund, M. R. (2014). Growth and cropping of’AU-Super’ or ‘Eaton’chestnut trees with ‘Little Giant’ interstem on AU-Cropper seedling rootstock. Journal of the American Pomological Society, 68(4), 190–197.

    Google Scholar 

  • Weidlich, W. H. (1978). A preliminary report on a method of biological control of the chestnut blight not involving the use of a hypovirulent strain of Endothia parasitica. In Proceedings of the American Chestnut Symposium, West Virginia University Books, Morgantown, West Virginia, (pp. 79–83).

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Acknowledgements

Authors are grateful to Dr. Carmen M. Medina MORA for proofreading the manuscript.

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Correspondence to Burak Akyüz.

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Akilli Şimşek, S., Akyüz, B., Katircioğlu, Y.Z. et al. Effectiveness and efficacy of superficial disinfectants to prevent mechanical transmission of Cryphonectria parasitica from chestnut scion woods. Eur J Plant Pathol 159, 131–138 (2021). https://doi.org/10.1007/s10658-020-02148-w

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