Key message

StMAPK11 overexpression promotes potato growth, physiological activities and photosynthesis under drought conditions.

Abstract

Mitogen-activated protein kinases (MAPKs) are import regulators of MAPK pathway in plants under drought condition. However, the critical role in potato (Solanum tuberosum L.) drought resistance is not fully understood. In this study, we aimed to explore the role of StMAPK11 under drought stress. The result of RT-qPCR for assay of StMAPKs expression demonstrated that 15 StMAPKs were differentially expressed in leaves, flowers, petioles, stamens, pistils, stems, stolons, roots, tubers and tuber peels of potato. StMAPKs was dynamically modulated by abiotic stresses and plant hormone treatments, and StMAPK11 was apparently up-regulated under drought conditions. Therefore, the vectors pCPB-StMAPK11 and pCPBI121-miRmapk11 for over-expression and down-regulation of StMAPK11 were constructed, respectively, and introduced into potato cultivar Atlantic. The result showed that StMAPK11 promoted potato growth under drought conditions, as well as the physiological activities evidenced by changes in SOD, CAT and POD activity and H₂O₂, proline and MDA content. StMAPK11 up-regulation intensified drought resistance of potato plant by elevating antioxidant activities and photosynthesis. Moreover, we consolidated the protective role of StMAPK11 in tobacco and Arabidopsis against drought stress. The result could provide new insights into the function of StMAPK11 in drought response and its possible mechanisms.

中文翻译：

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有丝分裂原活化蛋白激酶11（MAPK11）在干旱条件下维持马铃薯植物的生长和光合作用

关键信息

StMAPK11 过表达促进干旱条件下马铃薯的生长、生理活动和光合作用。

抽象的

丝裂原活化蛋白激酶 (MAPKs) 是干旱条件下植物 MAPK 通路的输入调节因子。然而，马铃薯 ( Solanum tuberosum L.) 抗旱性中的关键作用尚不完全清楚。在这项研究中，我们旨在探索StMAPK11在干旱胁迫下的作用。用RT-qPCR检测StMAPKs表达的结果表明，15个StMAPKs在马铃薯的叶、花、叶柄、雄蕊、雌蕊、茎、匍匐茎、根、块茎和块茎皮中差异表达。StMAPKs受到非生物胁迫和植物激素处理的动态调节，StMAPK11在干旱条件下明显上调。因此，分别构建了用于StMAPK11过表达和下调的载体pCPB-StMAPK11和pCPBI121-miRmapk11 ，并将其引入马铃薯栽培品种Atlantic。结果表明，StMAPK11促进了干旱条件下马铃薯的生长，以及SOD、CAT和POD活性以及H ₂ O ₂、脯氨酸和MDA含量的变化证明了马铃薯的生理活性。StMAPK11上调通过提高抗氧化活性和光合作用增强马铃薯植物的抗旱性。此外，我们巩固了StMAPK11在烟草和拟南芥中的保护作用对抗干旱胁迫。该结果可以为StMAPK11在干旱响应中的功能及其可能的机制提供新的见解。