Abstract
Key message
StMAPK11 overexpression promotes potato growth, physiological activities and photosynthesis under drought conditions.
Abstract
Mitogen-activated protein kinases (MAPKs) are import regulators of MAPK pathway in plants under drought condition. However, the critical role in potato (Solanum tuberosum L.) drought resistance is not fully understood. In this study, we aimed to explore the role of StMAPK11 under drought stress. The result of RT-qPCR for assay of StMAPKs expression demonstrated that 15 StMAPKs were differentially expressed in leaves, flowers, petioles, stamens, pistils, stems, stolons, roots, tubers and tuber peels of potato. StMAPKs was dynamically modulated by abiotic stresses and plant hormone treatments, and StMAPK11 was apparently up-regulated under drought conditions. Therefore, the vectors pCPB-StMAPK11 and pCPBI121-miRmapk11 for over-expression and down-regulation of StMAPK11 were constructed, respectively, and introduced into potato cultivar Atlantic. The result showed that StMAPK11 promoted potato growth under drought conditions, as well as the physiological activities evidenced by changes in SOD, CAT and POD activity and H2O2, proline and MDA content. StMAPK11 up-regulation intensified drought resistance of potato plant by elevating antioxidant activities and photosynthesis. Moreover, we consolidated the protective role of StMAPK11 in tobacco and Arabidopsis against drought stress. The result could provide new insights into the function of StMAPK11 in drought response and its possible mechanisms.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We thank Rongkai Wang (Bioediates Technology Corporation, Shanxi, China) for providing pART-CAM and pCAM35S-GFP expression vector.
Funding
This Research Program Sponsored by Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University (No.GSCS-2019-Z03) and National Natural Science Foundation of China (No.31960444).
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XZ, NZ and HS planned and designed the research. XZ, XL, SL, JY, XH and FW, collected the data. XZ, NZ, XL, SL and JY analyzed the data. XZ, NZ, XL and HS drafted the manuscript.
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299_2020_2645_MOESM4_ESM.tif
Supplementary Fig. 1 The antigen and antibody were prepared using Escherichia coli BL-21 strain and rabbit. PAGE analysis and Coomassie brilliant blue staining were used to detect (a) expression of recombinant protein StMAPK11. M, protein marker; 1, Protein extract from BL-21 stain without transfection. 2, Protein extract from the transfected BL-21 without IPTG induction; 3, IPTG induction at 16℃; 4, IPTG induction with 8 mol/L urea denaturation at 16oC; 5, IPTG induction at 30℃; 6, IPTG induction with 8 mol/L urea denaturation at 30℃; 7, IPTG induction at 37℃; 8, IPTG induction with 8 mol/L urea denaturation at 37℃; (b) affinity purification of recombinant protein StMAPK11. M, protein marker; 1, Antigen from BL-21 transfected with StMAPK11 with IPTG induction at 37℃ and denaturation in 4 mol/L urea. (c) WB verification of antibodies. M, Protein marker; 1-3, serum antibody titer is 1:32,000, and sample volume is 0.5 μg, 1 μg and 2 μg, respectively; 4-6, serum antibody titer is 1:64,000, and sample volume is 0.5 μg, 1 μg and 2 μg, respectively (TIF 997 KB)
299_2020_2645_MOESM5_ESM.tif
Supplementary Fig. 2 Potato plants were transfected with the recombinant plasmids designed for down-regulating or up-regulating StMAPK11 mRNA. PCR products were amplified from the plants (Lane 1-13) transformed with the recombinant plasmids (a) pCPB-StMAPK11 and (b) pCPBI121-miRmapk11 carrying NPT-II gene. M, DNA molecular weight standard DL2000; P, positive control; NT, non-transformed control; CK, control; NPT-II, 676 bp (TIF 7010 KB)
299_2020_2645_MOESM6_ESM.tif
Supplementary Fig. 3 Nicotiana tabacum (tobacco) and Arabidopsis thaliana (Arabidopsis) were transformed with the recombinant plasmids designed for up-regulating StMAPK11. PCR products were synthesized from (a) tobacco (Lane 1-16) and (b) Arabidopsis (Lane 1-13) transformed with pART-CAM-StMAPK11 plasmid. M, DNA molecular weight standard DL2000; P, positive control; WT, wild type; CK, control; PCR products, 415 bp and 728 bp (TIF 6947 KB)
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Zhu, X., Zhang, N., Liu, X. et al. Mitogen-activated protein kinase 11 (MAPK11) maintains growth and photosynthesis of potato plant under drought condition. Plant Cell Rep 40, 491–506 (2021). https://doi.org/10.1007/s00299-020-02645-6
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DOI: https://doi.org/10.1007/s00299-020-02645-6