Acinetobacter species are gram-negative, non-fermenting bacteria with coccobacilli morphology. The bacteria are found ubiquitously and have the ability to occupy niches which include environmental sites, animals, and humans. The original purpose of this study was to determine if painted turtles ( Chrysemys picta ) living in the wild in Western Wisconsin were colonized with carbapenem-resistant bacteria. Fecal samples from ten turtles were examined for carbapenem-resistant bacteria. None of the isolates were found to be carbapenem resistant by antimicrobial susceptibility testing. However, all the isolates were resistant to other β-lactams and chloramphenicol classes of antimicrobials. One isolate, Acinetobacter oleivorans strain PT8, was selected for additional characterization, including whole-genome sequencing (WGS). Strain PT8 is capable of degrading biodiesel, forming biofilms, and has a putative type 6 gene cluster. Finally, the taxonomic position of the available whole-genome sequences of 25 A. oleivorans genomes from purified isolates was determined.

中文翻译：

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从彩龟 (Chrysemys picta) 的粪便材料中分离出的生物柴油降解嗜油不动杆菌菌株 PT8 的表征

不动杆菌属是革兰氏阴性、非发酵细菌，具有球杆菌形态。这种细菌无处不在，并且能够占据包括环境场所、动物和人类在内的生态位。这项研究的最初目的是确定生活在威斯康星州西部野外的锦龟 (Chrysemys picta) 是否定植了耐碳青霉烯的细菌。检查了 10 只海龟的粪便样本中是否存在耐碳青霉烯类细菌。通过抗微生物药敏试验，没有发现分离株对碳青霉烯类耐药。然而，所有分离株都对其他 β-内酰胺类和氯霉素类抗菌剂具有抗性。选择一种分离物，即食油不动杆菌菌株 PT8，进行额外的表征，包括全基因组测序 (WGS)。菌株 PT8 能够降解生物柴油，形成生物膜，并具有推定的 6 型基因簇。最后，确定了来自纯化分离株的 25 个 A. oleivorans 基因组的可用全基因组序列的分类位置。