Owing to develop the utilization of biowaste materials and minimize the usage of plastic materials, Orange peel Powder (OPP) biowaste is chosen as filler material along with Polyvinyl Alcohol (PVA) as a matrix to form biocomposite films. To stretch its applications to antibacterial applications the metal nanoparticles were incorporated by the in-situ generation of Ag by reducing the various concentration of the aqueous solution of AgNO₃ to fabricate novel PVA/OPP/(1 mM to 5 mM)AgNPs hybrid biocomposite films. The fabricated biofilms were undergone the antibacterial test, mechanical test, and characterized by FESEM, FTIR, XRD & thermal analysis. The FESEM images clarify the homogenous distribution of filler materials in the PVA matrix and binding between the filler materials & matrices. FT-IR spectrum illustrates, there is no functional group change in the films by the inclusion of AgNPs as compare to the PVA/OPP films and indicates the strong adhesion and well dispersion of filler materials. XRD patterns confirm the presence of Ag and accentuated the crystallite size of generated AgNPs in the films as 23.44 nm, 25.59 nm, 26.25 nm, 28.17 nm, and 28.42 nm. Thermal analysis of the films shows improved thermal stability as well as glass transition temperature of the composite films included with AgNPs, also the considerable increase in the tensile strength and tensile modulus of the PVA/OPP/AgNPs films as compared to the neat PVA, PVA/OPP films. As compare to neat PVA and PVA/OPP films, with the increase of concentration of AgNO₃ source solution antibacterial activity of the PVA/OPP/AgNPs films increases against gram-negative and gram-positive bacteria. With the above-improved results by the inclusion of Ag nanoparticles, this hybrid biocomposite films can be utilized in food processing industries

由于发展了生物废物材料的利用并最大程度地减少了塑料材料的使用，桔皮粉（OPP）生物废物被选为填充材料，而聚乙烯醇（PVA）作为基质形成生物复合膜。为了将其应用扩展到抗菌应用，通过降低AgNO ₃水溶液的各种浓度，通过原位生成Ag引入了金属纳米颗粒制备新型PVA / OPP /（1 mM至5 mM）AgNPs混合生物复合膜。制备的生物膜经过了抗菌测试，机械测试，并通过FESEM，FTIR，XRD和热分析进行了表征。FESEM图像阐明了填充材料在PVA基质中的均匀分布以及填充材料与基质之间的结合。FT-IR光谱表明，与PVA / OPP薄膜相比，通过包含AgNPs薄膜中的官能团没有变化，并且表明填料具有很强的粘合力和良好的分散性。XRD图谱证实了Ag的存在，并增强了膜中产生的AgNP的微晶尺寸为23.44nm，25.59nm，26.25nm，28.17nm和28.42nm。薄膜的热分析表明，AgNPs包含的复合薄膜的热稳定性和玻璃化转变温度得到改善，与纯PVA，PVA相比，PVA / OPP / AgNPs薄膜的拉伸强度和拉伸模量也显着提高/ OPP电影。与纯PVA和PVA / OPP薄膜相比，随着AgNO浓度的增加PVA / OPP / AgNPs膜的_3种源溶液的抗菌活性均对革兰氏阴性和革兰氏阳性细菌增加。通过包含银纳米颗粒，具有以上改进的结果，这种杂化生物复合膜可用于食品加工行业