Engineered cartilage has several applications in treating cartilage ossification, however, its use is restricted clinically. We explored the feasibility of engineered cartilage in constructing tissues using gelatin/curcumin nano-fiber membranes as scaffolds in subcutaneous models. We constructed cartilage with gelatin nano-fiber membrane (control group) and gelatin/curcumin nano-fiber membrane (experimental group) as scaffolds. After the material was implanted into the back of BALB/c mice, gross view observation was performed. Histological examination was performed 3 and 12 weeks after implantation in vivo, and cartilage formation at different time points was compared. Gross observation showed that compared to the control group, the vascularization of nearby tissues in the experimental group was significantly inhibited. The Scanning electron microscope observation showed that the chondrocytes in both groups adhered well. The growth curve of the chondrocytes showed that curcumin had no significant effect on cell growth. Histological observation showed that the cell-material complexes in both groups had cartilage lacuna formation at 3 and 12 weeks. However, compared with that of the control group, the experimental group showed obvious absorption and thicker cartilage matrix with more homogenization. Gelatin/curcumin scaffolds were successfully used to construct engineered cartilage tissues in subcutaneous animal models. Our findings demonstrate that curcumin-loaded scaffolds have great clinical applications.

工程软骨在治疗软骨骨化方面有多种应用，但其在临床上的使用受到限制。我们探索了工程软骨在使用明胶/姜黄素纳米纤维膜作为皮下模型支架构建组织中的可行性。我们以明胶纳米纤维膜（对照组）和明胶/姜黄素纳米纤维膜（实验组）为支架构建软骨。将材料植入BALB/c小鼠背部后，进行大体观察。在体内植入后3周和12周进行组织学检查，比较不同时间点的软骨形成情况。大体观察显示，与对照组相比，实验组附近组织的血管化明显受到抑制。扫描电镜观察显示，两组软骨细胞均贴壁良好。软骨细胞的生长曲线表明姜黄素对细胞生长没有显着影响。组织学观察显示，两组细胞-材料复合物在第3周和第12周均有软骨缺损形成。然而，与对照组相比，实验组表现出明显的吸收和更厚的软骨基质，更均匀。明胶/姜黄素支架已成功用于在皮下动物模型中构建工程软骨组织。我们的研究结果表明，姜黄素支架具有很好的临床应用。软骨细胞的生长曲线表明姜黄素对细胞生长没有显着影响。组织学观察显示，两组细胞-材料复合物在第3周和第12周均有软骨缺损形成。然而，与对照组相比，实验组表现出明显的吸收和更厚的软骨基质，更均匀。明胶/姜黄素支架已成功用于在皮下动物模型中构建工程软骨组织。我们的研究结果表明，姜黄素支架具有很好的临床应用。软骨细胞的生长曲线表明姜黄素对细胞生长没有显着影响。组织学观察显示，两组细胞-材料复合物在第3周和第12周均有软骨缺损形成。然而，与对照组相比，实验组表现出明显的吸收和更厚的软骨基质，更均匀。明胶/姜黄素支架已成功用于在皮下动物模型中构建工程软骨组织。我们的研究结果表明，姜黄素支架具有很好的临床应用。实验组表现出明显的吸收和更厚的软骨基质，更均匀。明胶/姜黄素支架已成功用于在皮下动物模型中构建工程软骨组织。我们的研究结果表明，姜黄素支架具有很好的临床应用。实验组表现出明显的吸收和更厚的软骨基质，更均匀。明胶/姜黄素支架已成功用于在皮下动物模型中构建工程软骨组织。我们的研究结果表明，姜黄素支架具有很好的临床应用。