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Using gelatin/curcumin nano-fiber membranes as scaffolds in a subcutaneous model for tissue engineered cartilages

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Abstract

Engineered cartilage has several applications in treating cartilage ossification, however, its use is restricted clinically. We explored the feasibility of engineered cartilage in constructing tissues using gelatin/curcumin nano-fiber membranes as scaffolds in subcutaneous models. We constructed cartilage with gelatin nano-fiber membrane (control group) and gelatin/curcumin nano-fiber membrane (experimental group) as scaffolds. After the material was implanted into the back of BALB/c mice, gross view observation was performed. Histological examination was performed 3 and 12 weeks after implantation in vivo, and cartilage formation at different time points was compared. Gross observation showed that compared to the control group, the vascularization of nearby tissues in the experimental group was significantly inhibited. The Scanning electron microscope observation showed that the chondrocytes in both groups adhered well. The growth curve of the chondrocytes showed that curcumin had no significant effect on cell growth. Histological observation showed that the cell-material complexes in both groups had cartilage lacuna formation at 3 and 12 weeks. However, compared with that of the control group, the experimental group showed obvious absorption and thicker cartilage matrix with more homogenization. Gelatin/curcumin scaffolds were successfully used to construct engineered cartilage tissues in subcutaneous animal models. Our findings demonstrate that curcumin-loaded scaffolds have great clinical applications.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (81871502), Youth program of National Natural Science Foundation of China(8180080719) and The National Key Research and Development Program of China (2017YFC1103900). The authors appreciate technical support and other assistance from Junming Lu, Xiansong Wang and Lijuan Zong.

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All authors declare no competing financial interests.

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Author notes

  1. Bo kyoung Kang and Zheyuan Yu contributed equally to this work and should be viewed as co-first authors to this manuscript.

Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China

    Bo Kyoung Kang, Zheyuan Yu, Taoran Jiang, Yoong-hun Shim, Jiaman Gao, Guangdong Zhou & Dejun Cao

  2. Shanghai Key Laboratory of Tissue Engineering, Shanghai, 200011, China

    Bo Kyoung Kang, Weiming Chen & Guangdong Zhou

  3. National Tissue Engineering Center of China, Shanghai, 200241, China

    Weiming Chen & Guangdong Zhou

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  1. Bo Kyoung Kang
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  2. Zheyuan Yu
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  7. Guangdong Zhou
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Contributions

BkK contributed to the conception and design, follow-up and data collection, analysis and interpretation of data of this study and wrote this manuscript. ZY contributed to the conception and design, acquisition of data, analysis and interpretation of data of this study and the draft and revision of this manuscript for important intellectual content. WC, TJ, Y-hS, JG contributed to the acquisition of data of this study and revision of the article. GZ contributed to the analysis and interpretation of data of this study. DC contributed to the concept and design and acquisition of data of this study. All authors give final approval of this version to be published.

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Correspondence to Guangdong Zhou or Dejun Cao.

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Kang, B.K., Yu, Z., Chen, W. et al. Using gelatin/curcumin nano-fiber membranes as scaffolds in a subcutaneous model for tissue engineered cartilages. Cell Tissue Bank 22, 443–451 (2021). https://doi.org/10.1007/s10561-020-09892-7

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  • DOI: https://doi.org/10.1007/s10561-020-09892-7

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