Objectives The co-encapsulation of bioactive peptides obtained from degradation of chicken feathers and flexirubin-type pigment produced by Chryseobacterium sp. kr6 into phosphatidylcholine liposomes was investigated. Results Control empty liposomes showed mean diameter of 168.5 nm, varying to 185.4, 102.0 and 98.5 nm after the encapsulation of peptides, pigment and their co-encapsulation, respectively. Control liposomes presented zeta potential of − 20.9 mV, while the formulations containing the bioactive compounds showed values of − 30 mV or higher in magnitude. Infrared analysis revealed typical spectra for phosphatidylcholine, suggesting that no new chemical bonds were formed after encapsulation. ABTS radical scavenging assay showed that the antioxidant activity of the compounds was maintained after encapsulation. Conclusions Feather waste can be a valuable substrate for simultaneous production of antioxidant peptides and pigment by Chryseobacterium sp. kr6, and their encapsulation into liposomes may be a suitable alternative for delivery of these natural antioxidants.

中文翻译：

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羽毛微生物转化为抗氧化肽和色素及其脂质体封装

目的 将鸡羽毛降解得到的生物活性肽与金黄色杆菌产生的柔红素型色素进行共包封。研究了 kr6 进入磷脂酰胆碱脂质体的过程。结果 对照空脂质体的平均直径为 168.5 nm，在肽、色素和它们的共包封后分别变为 185.4、102.0 和 98.5 nm。对照脂质体的 zeta 电位为 − 20.9 mV，而含有生物活性化合物的制剂的数值为 − 30 mV 或更高。红外分析揭示了磷脂酰胆碱的典型光谱，表明封装后没有形成新的化学键。ABTS 自由基清除试验表明，封装后化合物的抗氧化活性得以保持。结论 羽毛废料可以作为金黄色杆菌同时生产抗氧化肽和色素的有价值的底物。kr6，并将它们封装在脂质体中可能是递送这些天然抗氧化剂的合适替代品。