Objectives To select a microbial consortium from intertidal sludge and evaluate its ability to convert crude glycerol from biodisel to high value-added products such as 1,3-propanediol (1,3-PDO) and lactic acid (LA). Results A microbial consortium named CJD-S was selected from intertidal sludge and exhibited excellent performance for the conversion of crude glycerol to 1,3-PDO and LA. The composition of CJD-S was determined to be 85.99% Enterobacteriaceae and 13.75% Enterococcaceae by 16S rRNA gene amplicon high-throughput sequencing. In fed-batch fermentation with crude glycerol under nonsterile conditions, the highest concentrations of 1,3-PDO and LA were 41.47 g/L and 45.86 g/L, respectively. Conclusions The selected microbial consortium, CJD-S, effectively converted crude glycerol to 1,3-PDO and LA under nonsterile conditions and can contribute to the sustainable development of the biodiesel industry.

中文翻译：

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潮间带污泥微生物聚生体从粗甘油生产1,3-丙二醇和乳酸

目的 从潮间带污泥中选择微生物群落并评估其将生物柴油中的粗甘油转化为高附加值产品如 1,3-丙二醇 (1,3-PDO) 和乳酸 (LA) 的能力。结果从潮间带污泥中筛选出一个名为CJD-S的微生物群落，在将粗甘油转化为1,3-PDO和LA方面表现出优异的性能。通过16S rRNA基因扩增子高通量测序确定CJD-S的组成为85.99%肠杆菌科和13.75%肠球菌科。在非无菌条件下用粗甘油分批补料发酵中，1,3-PDO 和 LA 的最高浓度分别为 41.47 g/L 和 45.86 g/L。结论 选定的微生物聚生体 CJD-S 有效地将粗甘油转化为 1，