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Production of 1,3-propanediol and lactic acid from crude glycerol by a microbial consortium from intertidal sludge

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Abstract

Objectives

To select a microbial consortium from intertidal sludge and evaluate its ability to convert crude glycerol from biodisel to high value-added products such as 1,3-propanediol (1,3-PDO) and lactic acid (LA).

Results

A microbial consortium named CJD-S was selected from intertidal sludge and exhibited excellent performance for the conversion of crude glycerol to 1,3-PDO and LA. The composition of CJD-S was determined to be 85.99% Enterobacteriaceae and 13.75% Enterococcaceae by 16S rRNA gene amplicon high-throughput sequencing. In fed-batch fermentation with crude glycerol under nonsterile conditions, the highest concentrations of 1,3-PDO and LA were 41.47 g/L and 45.86 g/L, respectively.

Conclusions

The selected microbial consortium, CJD-S, effectively converted crude glycerol to 1,3-PDO and LA under nonsterile conditions and can contribute to the sustainable development of the biodiesel industry.

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Data availability

The datasets generated during and analysed during the current study are available in the NCBI Sequence Read Archive (SRA) repository, https://dataview.ncbi.nlm.nih.gov/?search=SUB7972898&archive=sra.

Abbreviations

1,3-PDO:

1,3-Propanediol

LA:

Lactic acid

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Acknowledgements

The authors acknowledge the Key Lab of Biomass Energy and Materials Open Foundation, Jiangsu Province (No. JSBEM202019), the Liaoning Natural Science Foundation Program, China (No. 2019BS238), the Liaoning Key Laboratory of Additive Synthesis and Separation Foundation Program, China (No. 112381), and the Program for Excellent Talents of Science and Technology in Yingkou Institute of Technology.

Funding

This study was supported by the Key Lab of Biomass Energy and Materials Open Foundation, Jiangsu Province (No. JSBEM202019), the Liaoning Natural Science Foundation Program, China (No. 2019BS238), the Liaoning Key Laboratory of Additive Synthesis and Separation Foundation Program, China (No. 112381), and the Program for Excellent Talents of Science and Technology in Yingkou Institute of Technology.

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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by LLJ, FYL and WY. The first draft of the manuscript was written by LLJ and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Li-Li Jiang.

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Jiang, LL., Liu, FY., Yang, W. et al. Production of 1,3-propanediol and lactic acid from crude glycerol by a microbial consortium from intertidal sludge. Biotechnol Lett 43, 711–717 (2021). https://doi.org/10.1007/s10529-020-03063-0

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