Introduction

This study aimed to examine the bone-forming ability of medium-cross-linked recombinant collagen peptide (mRCP) particles developedbased on human collagen type I, contains an arginyl-glycyl-aspartic acid-rich motif, fabricated as bone filling material, compared to that of the autologous bone graft.

Methods

Calvarial bone defects were created in immunodeficient rats though a surgical procedure. The rats were divided into 2 groups: mRCP graft and tibia bone graft (bone graft). The bone formation potential of mRCP was evaluated by micro-computed tomography and hematoxylin-eosin staining at 1, 2, 3, and 4 weeks after surgery, and the data were analyzed and compared to those of the bone graft.

Results

The axial volume-rendered images demonstrated considerable bony bridging with the mRCP graft, but there was no significant difference in the bone volume and bone mineral density between the mRCP graft and bone graft at 4 weeks. The peripheral new bone density was significantly higher than the central new bone density and the bottom side score was significantly higher than the top side score at early stage in the regenerated bone within the bone defects.

Conclusion

These results indicate that mRCP has a high potential of recruiting osteogenic cells, comparable to that of autologous bone chips.

中文翻译：

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基于 I 型胶原的重组肽颗粒在大鼠颅盖骨缺损中的骨形成潜力

介绍

本研究旨在检查基于人 I 型胶原蛋白开发的中等交联重组胶原蛋白肽 (mRCP) 颗粒的骨形成能力，该颗粒含有富含精氨酰-甘氨酰-天冬氨酸的基序，制造为骨填充材料，与即自体骨移植。

方法

通过外科手术在免疫缺陷大鼠中产生颅骨缺损。将大鼠分为2组：mRCP移植物和胫骨骨移植物（骨移植物）。mRCP在术后1、2、3、4周通过微型计算机断层扫描和苏木精-伊红染色评估mRCP的成骨潜力，并与骨移植的数据进行分析和比较。

结果

轴向体积渲染图像显示与 mRCP 移植物有相当大的骨桥接，但在 4 周时，mRCP 移植物和骨移植物之间的骨体积和骨矿物质密度没有显着差异。骨缺损内再生骨早期外周新生骨密度明显高于中心新生骨密度，底侧评分明显高于顶侧评分。

结论

这些结果表明，mRCP 具有招募成骨细胞的高潜力，与自体骨片相当。