Bone formation potential of collagen type I-based recombinant peptide particles in rat calvaria defects

Highlights

• Bone formation potential of mRCP were comparable to that of autogenous bone.

• mRCP particles exhibit high new bone formation potential in the calvaria defect.

• Bone bridging was observed over the entire defect in mRCP graft at 4 weeks.

• mRCP has a high potential of recruiting osteogenic cells comparable to bone graft.

Abstract

Introduction

This study aimed to examine the bone-forming ability of medium-cross-linked recombinant collagen peptide (mRCP) particles developedbased on human collagen type I, contains an arginyl-glycyl-aspartic acid-rich motif, fabricated as bone filling material, compared to that of the autologous bone graft.

Methods

Calvarial bone defects were created in immunodeficient rats though a surgical procedure. The rats were divided into 2 groups: mRCP graft and tibia bone graft (bone graft). The bone formation potential of mRCP was evaluated by micro-computed tomography and hematoxylin-eosin staining at 1, 2, 3, and 4 weeks after surgery, and the data were analyzed and compared to those of the bone graft.

Results

The axial volume-rendered images demonstrated considerable bony bridging with the mRCP graft, but there was no significant difference in the bone volume and bone mineral density between the mRCP graft and bone graft at 4 weeks. The peripheral new bone density was significantly higher than the central new bone density and the bottom side score was significantly higher than the top side score at early stage in the regenerated bone within the bone defects.

Conclusion

These results indicate that mRCP has a high potential of recruiting osteogenic cells, comparable to that of autologous bone chips.