Lipase is an important commercial enzyme with unique and versatile biotechnological applications. This study was conducted to biosynthesize and characterizes alkaliphilic lipase by Exiguobacterium sp. strain AMBL-20T isolated from the glacial water samples of the northeastern (Gilgit-Baltistan) region of Pakistan. The isolated bacterium was identified as Exiguobaterium sp. strain AMBL-20T on the basis of morphological, biochemical, and phylogenetic analysis of 16S rRNA sequences with GenBank accession number MW229267. The bacterial strain was further screened for its lipolytic activity, biosynthesis, and characterization by different parameters with the aim of maximizing lipase activity. Results showed that 2% Olive oil, 0.2% peptone at 25 °C, pH 8, and 24 h of incubation time found optimal for maximum lipase production. The lipase enzyme was partially purified by ammonium sulphate precipitation and its activity was standardized at pH 8 under 30 °C temperature. The enzyme showed functional stability over a range of temperature and pH. Hence, extracellular alkaliphilic lipase from Exiguobacterium sp. is a potential candidate with extraordinary industrial applications, particularly in bio-detergent formulations.

中文翻译：

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新型耐低温的 Exiguobacterium sp. 产生碱性脂肪酶。从巴基斯坦东北部冰川分离的菌株 (AMBL-20)

脂肪酶是一种重要的商业酶，具有独特且多功能的生物技术应用。本研究旨在通过 Exiguobacterium sp. 生物合成和表征嗜碱脂肪酶。从巴基斯坦东北部（吉尔吉特-巴尔蒂斯坦）地区的冰川水样品中分离出菌株 AMBL-20T。分离出的细菌被鉴定为 Exiguobaterium sp。根据对 GenBank 登录号 MW229267 的 16S rRNA 序列进行形态学、生化和系统发育分析，菌株 AMBL-20T。进一步筛选细菌菌株的脂解活性、生物合成和不同参数的表征，目的是最大化脂肪酶活性。结果表明，在 25 °C、pH 8 和 24 小时的孵育时间下，2% 的橄榄油、0.2% 的蛋白胨最能产生最大的脂肪酶。脂肪酶通过硫酸铵沉淀部分纯化，其活性在 pH 8 和 30 °C 温度下标准化。该酶在一定温度和 pH 值范围内表现出功能稳定性。因此，来自 Exiguobacterium sp. 的细胞外嗜碱脂肪酶。是具有非凡工业应用的潜在候选者，特别是在生物洗涤剂配方中。