Analysis of the complete nucleotide sequence of the pBS72 plasmid from an environmental strain Bacillus subtilis 72 (102 254 bp) was used to characterize the genetic factors responsible for its vertical and horizontal gene transfer. Replicons similar to pBS72 were found to be present in B. subtilis isolated from various natural environments in Pakistan and the Netherlands (99% identity of the Rep proteins). The relaxase of the pBS72 plasmid, which is responsible for its conjugative transfer, was found to belong to the MobL family. A total of 142 open reading frames were revealed in the genome of the pBS72 plasmid, most of which (93) coded the synthesis of hypothetical polypeptides. Among the known proteins of the pBS72 plasmid genes in members of the genus Bacillus, the polypeptides were revealed, which were responsible for the interaction of bacterial cells with the environment, protection from foreign DNA and damage, as well as those involved in the cellular metabolism and carrying out regulatory functions. A system was developed in order to determine the role of the plasmid in activity of environmental and collection B. subtilis strains. In the presence of 13% NaCl, or at pH values of 4.5 and 11, survival of the environmental strain B. subtilis 72 and of the collection strain B. subtilis 168 was 4.33 and 13 times, 1.75 and 1.95 times, and 1.75 and 9.12 times higher, respectively. UV irradiation resulted in 2.25 times higher survival of B. subtilis 72 and 4.5 times higher survival of B. subtilis 168. The environmental strain B. subtilis 72 had more pronounced adaptive properties, which may indicate existence of additional genetic determinants in their chromosome; interaction of their products with the proteins of plasmid origin was probably responsible for higher resistance to increased osmolarity, high and low pH, and UV irradiation.

中文翻译：

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来自枯草芽孢杆菌环境分离株的 PBS72 质粒的分子遗传和功能分析

来自环境菌株枯草芽孢杆菌 72 (102 254 bp) 的 pBS72 质粒的完整核苷酸序列的分析用于表征负责其垂直和水平基因转移的遗传因素。发现与 pBS72 相似的复制子存在于从巴基斯坦和荷兰的各种自然环境中分离的枯草芽孢杆菌中（Rep 蛋白的 99% 同一性）。负责其接合转移的 pBS72 质粒的松弛酶被发现属于 MobL 家族。在 pBS72 质粒的基因组中总共揭示了 142 个开放阅读框，其中大部分 (93) 编码假设多肽的合成。在芽孢杆菌属成员中 pBS72 质粒基因的已知蛋白质中，多肽被揭示，它们负责细菌细胞与环境的相互作用，防止外来 DNA 和损伤，以及参与细胞代谢和执行调节功能的细胞。开发了一个系统以确定质粒在环境和收集枯草芽孢杆菌菌株的活性中的作用。在 13% NaCl 存在下，或在 4.5 和 11 的 pH 值下，环境菌株 B. subtilis 72 和收集菌株 B. subtilis 168 的存活率分别是 4.33 和 13 倍、1.75 和 1.95 倍以及 1.75 和 9.12分别高出数倍。紫外线照射使 B. subtilis 72 的存活率提高了 2.25 倍，B. subtilis 168 的存活率提高了 4.5 倍。环境菌株 B. subtilis 72 具有更显着的适应性，这可能表明其染色体中存在额外的遗传决定因素；他们的产品与质粒来源的蛋白质的相互作用可能是对增加的渗透压、高和低 pH 值以及紫外线照射具有更高抵抗力的原因。