Applied Biochemistry and Microbiology ( IF 1.0 ) Pub Date : 2020-12-01 , DOI: 10.1134/s0003683820080086 L. E. Ryabchenko , T. E. Leonova , T. E. Shustikova , T. V. Gerasimova , T. A. Ivankova , K. V. Sidorenko , A. S. Yanenko
Abstract
The psefdh_D221Q gene encodes a mutant formate dehydrogenase from Pseudomonas (P-seFDG_D221Q), which catalyzes formate oxidation with the simultaneous formation of NADPH. It is expressed in cells of lysine-producing Corynebacterium glutamicum strains. The psefdh_D221Q gene was introduced into C. glutamicum strains as part of an autonomous plasmid or was integrated into the chromosome with the simultaneous inactivation of the host formate-dehydrogenase genes. It was shown that the C. glutamicum strains with NADP+-dependent formate dehydrogenase have an increased level of L-lysine synthesis in the presence of formate if their own formate dehydrogenase is inactivated.
中文翻译:
假单胞菌NADPH +依赖的甲酰胺脱氢酶基因的表达增加了谷氨酸棒杆菌中赖氨酸的产生。
摘要
所述psefdh _D221Q基因编码的突变型甲酸脱氢酶从假单胞菌(P-seFDG_D221Q),其催化与NADPH同时形成甲酸盐氧化。它在产生赖氨酸的谷氨酸棒杆菌菌株的细胞中表达。所述psefdh _D221Q基因导入谷氨酸棒杆菌菌株作为自主质粒的一部分或整合入与主机甲酸脱氢酶的基因的失活同时染色体。结果表明,带有NADP +的谷氨酸棒杆菌菌株如果自身存在的甲酸脱氢酶被灭活,则依赖于甲酸的氢脱氢酶在甲酸存在下具有增加的L-赖氨酸合成水平。