Abstract
The psefdh_D221Q gene encodes a mutant formate dehydrogenase from Pseudomonas (P-seFDG_D221Q), which catalyzes formate oxidation with the simultaneous formation of NADPH. It is expressed in cells of lysine-producing Corynebacterium glutamicum strains. The psefdh_D221Q gene was introduced into C. glutamicum strains as part of an autonomous plasmid or was integrated into the chromosome with the simultaneous inactivation of the host formate-dehydrogenase genes. It was shown that the C. glutamicum strains with NADP+-dependent formate dehydrogenase have an increased level of L-lysine synthesis in the presence of formate if their own formate dehydrogenase is inactivated.
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ACKNOWLEDGMENTS
This work was carried out on the equipment of the Multipurpose Scientific Installation of All-Russia Collection of Industrial Microorganisms of the Kurchatov Institute National Resource Center (GOSNIIGENETIKA).
Funding
This work was financially supported by the Ministry of Education and Science of Russia (Unique Project Identifier RFMEFI61017X0011).
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Translated by I. Gordon
Abbreviations: CL—culture liquid; FDH–formate dehydrogenase; NADPH—nicotinamide-β-adenine dinucleotide phosphate, reduced; OD—optical density; PdeFDH_D221Q—NADPH+-dependent FDH with the D221Q mutation.
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Ryabchenko, L.E., Leonova, T.E., Shustikova, T.E. et al. Expression of the NADPH+-Dependent Formate-Dehydrogenase Gene from Pseudomonas Increases Lysine Production in Corynebacterium glutamicum . Appl Biochem Microbiol 56, 828–836 (2020). https://doi.org/10.1134/S0003683820080086
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DOI: https://doi.org/10.1134/S0003683820080086