Abstract

In recent years, mycelial fungi have faced competition from recombinant yeast as producers of feed enzymes. An intensive study on genetic diversity identified the yeast genes encoding feed enzymes, the specific activity of which is much higher than that in mycelial fungi. In addition, these genes were expressed in yeast much more efficiently than in mycelial fungi. The use of yeast recombinant producers allowed the expansion of the production of a line of industrial enzymes with a significant reduction in their cost. The advantages of yeast producers of recombinant enzymes include the ability to obtain monoenzymes, which are part of various enzyme complexes used for different purposes. Pichia pastoris methylotrophic yeast is the most attractive subject for the creation of recombinant protein-producing strains.

中文翻译：

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在饲料酶工业生产者中使用甲基营养酵母的前景

摘要

近年来，作为饲料酶的生产者，菌丝真菌面临着来自重组酵母的竞争。对遗传多样性的深入研究确定了编码饲料酶的酵母基因，其比活性远高于菌丝真菌。另外，这些基因在酵母中的表达比在菌丝真菌中的表达要有效得多。酵母重组生产商的使用使工业酶系列的生产得以扩大，其成本大大降低。重组酶酵母生产商的优势包括获得单酶的能力，这些酶是用于不同目的的各种酶复合物的一部分。巴斯德毕赤酵母甲基营养型酵母是产生重组蛋白生产菌株的最有吸引力的主题。