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HbWRKY27, a group IIe WRKY transcription factor, positively regulates HbFPS1 expression in Hevea brasiliensis
Scientific Reports ( IF 3.8 ) Pub Date : 2020-11-26 , DOI: 10.1038/s41598-020-77805-5
Long Qu 1, 2 , Hui-Liang Li 2 , Dong Guo 2 , Ying Wang 2 , Jia-Hong Zhu 2 , Li-Yan Yin 1 , Shi-Qing Peng 2
Affiliation  

Farnesyl pyrophosphate synthase (FPS) is a key enzyme that catalyzes the formation of farnesyl pyrophosphate, the main initiator for rubber chain initiation in Hevea brasiliensis Muell. Arg. The transcriptional regulatory mechanisms of the FPS gene still not well understood. Here, a WRKY transcription factor designated HbWRKY27 was obtained by screening the latex cDNA library applied the HbFPS1 promoter as bait. HbWRKY27 interacted with the HbFPS1 promoter was further identified by individual Y1H and EMSA assays. HbWRKY27 belongs to group IIe WRKY subfamily which contains a typical WRKY domain and C-X5-CX23-HXH motif. HbWRKY27 was localized to the nucleus. HbWRKY27 predominantly accumulated in latex. HbWRKY27 was up-regulated in latex by ethrel, salicylic acid, abscisic acid, and methyl jasmonate treatment. Transient expression of HbWRKY27 led to increasing the activity of the HbFPS1 promoter in tobacco plant, suggesting that HbWRKY27 positively regulates the HbFPS1 expression. Taken together, an upstream transcription factor of the key natural rubber biosynthesis gene HbFPS1 was identified and this study will provide novel transcriptional regulatory mechanisms of the FPS gene in Hevea brasiliensis.



中文翻译:


HbWRKY27 是一种 IIe WRKY 转录因子,可正向调节巴西橡胶树中的 HbFPS1 表达



法尼基焦磷酸合酶 (FPS) 是催化法尼基焦磷酸形成的关键酶,法尼基焦磷酸是橡胶链起始的主要引发剂。精氨酸。 FPS 基因的转录调控机制仍不清楚。在此,通过筛选以HbFPS1启动子为诱饵的乳胶cDNA文库,获得了命名为HbWRKY27的WRKY转录因子。通过单独的 Y1H 和 EMSA 测定进一步鉴定了 HbWRKY27 与HbFPS1启动子的相互作用。 HbWRKY27属于IIe组WRKY亚家族,包含典型的WRKY结构域和C-X5-CX23-HXH基序。 HbWRKY27 定位于细胞核。 HbWRKY27主要积聚在乳胶中。经过乙烯利、水杨酸、脱落酸和茉莉酸甲酯处理后,乳胶中的HbWRKY27上调。 HbWRKY27 的瞬时表达导致烟草植物中HbFPS1启动子的活性增加,表明 HbWRKY27 正向调节HbFPS1的表达。总之,鉴定了天然橡胶生物合成关键基因HbFPS1的上游转录因子,本研究将为巴西橡胶树FPS 基因提供新的转录调控机制。

更新日期:2020-11-27
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