The present study aims at the amelioration of chromium Cr(VI) toxicity using ethylenediaminetetraacetic acid (EDTA), and to understand the interactive effects of Cr(VI) and EDTA with respect to seedling growth, lipid peroxidation as assessed from malondialdehyde, pigments and antioxidative enzymes in Hordeum vulgare L. Following multivariate statistical techniques were used to study binary interactions between Cr(VI) and EDTA: 2-way ANOVA, Tukey’s multiple comparison test, multiple regression with interaction between Cr an EDTA, beta coefficients, path analysis and non-metric multidimensional scaling (NMDS). The present study revealed that the EDTA decreases lipid peroxidation induced by Cr(VI) and ameliorates the antioxidative defence system and pigment constitution of seedlings grown in Cr(VI) containing media. EDTA–Cr(VI) interaction decreased the Cr content in the seedlings which may be attributed to the chelating effect of EDTA. The root and shoot bioconcentration factors, the ratio of Cr content in the plant to that in the medium, were decreased by addition of EDTA to Cr(VI), indicating a decrease in the uptake of Cr by the seedlings from the medium. NMDS revealed that the ranking of the studied parameters is maintained by ordination on two axes. The study established that EDTA is antagonistic to Cr(VI) induced biochemical toxicity, and improves the antioxidative defence system, increases the chlorophyll content, and decreases Cr uptake in barley seedlings.

本研究旨在使用乙二胺四乙酸 (EDTA) 改善铬 Cr(VI) 的毒性，并了解 Cr(VI) 和 EDTA 在幼苗生长、脂质过氧化方面的相互作用，如从丙二醛、色素和抗氧化大麦中的酶L. 以下多元统计技术用于研究 Cr(VI) 和 EDTA 之间的二元相互作用：2-way ANOVA、Tukey 的多重比较检验、Cr 和 EDTA 之间相互作用的多元回归、β 系数、路径分析和非度量多维标度(NMDS)。本研究表明，EDTA 可降低 Cr(VI) 诱导的脂质过氧化，并改善在含 Cr(VI) 培养基中生长的幼苗的抗氧化防御系统和色素构成。EDTA-Cr(VI) 相互作用降低了幼苗中的 Cr 含量，这可能是由于 EDTA 的螯合作用。根和芽的生物浓度因子，即植物中铬含量与培养基中铬含量的比率，通过向 Cr(VI) 中添加 EDTA 来降低，表明幼苗从培养基中吸收 Cr 的减少。NMDS 显示，研究参数的排序是通过两个轴上的排序来维护的。研究表明，EDTA 可以拮抗 Cr(VI) 诱导的生化毒性，改善抗氧化防御系统，增加叶绿素含量，降低大麦幼苗对 Cr 的吸收。