Poultry spermatozoa are prone to oxidative damage and adversely affect their fertility. Bioactive constituents of citrus fruit confer antioxidant enrichment on its juice and are candidates to combat oxidative load in poultry semen. Computer-assisted semen analyser and oxidative status were used to evaluate the potency of two tropical citrus varieties (sweet orange and tangerine) as natural diluents for rooster semen. Fresh and ripe sweet orange and tangerine fruits were obtained and processed into juices using a standard protocol and included in dextrose saline at 0%, 10%, 20%, 30%, 40%, 50% and 60% as semen diluent. Semen pool from 30 breeder roosters of 35–40 weeks of age was allotted randomly in triplicates to the different fruit juice-dextrose at 1:2 dilution rate and evaluated for 5 h at room temperature. Diluted semen, according to treatments, was evaluated for sperm kinetics using a computer-assisted sperm analyser, and seminal plasma was assayed for lipid peroxidation and total antioxidant activity. The result obtained shows that tangerine and sweet orange juice inclusion significantly (p < 0.05) enhance progressive spermatozoa motility and semen kinetics compared favourably with undiluted semen. The diluents had a proportionate increase in antioxidant activity with juice inclusion at 0 h and the antioxidant activity of 40%, 50% and 60% fruit juice-based diluents was higher than undiluted semen at 5 h. There was a reduced lipid peroxidation in juice inclusive diluents and increase lipid peroxidation rate in undiluted semen and 0% juice inclusion. In conclusion, tangerine and sweet orange juice inhibit lipid peroxidation in rooster semen and enhance progressive spermatozoa motility and maintain rooster semen kinetics.

家禽精子容易受到氧化损伤，并对其生育能力产生不利影响。柑橘类水果的生物活性成分可在其果汁中提供抗氧化剂富集，是对抗家禽精液中氧化负荷的候选者。使用计算机辅助的精液分析仪和氧化状态来评估两种热带柑橘品种（甜橙和橘子）作为公鸡精液的天然稀释剂的效力。获得新鲜和成熟的甜橙和橘子水果，并使用标准方案将其加工成果汁，并以0％，10％，20％，30％，40％，50％和60％的精液稀释剂包含在葡萄糖盐水中。将30只35-40周龄种鸡的精液库一式三份随机分配给不同的果汁-葡萄糖（稀释度为1：2），并在室温下评估5小时。精液稀释 根据治疗方法，使用计算机辅助的精子分析仪评估精子动力学，并分析精浆中脂质的过氧化和总抗氧化活性。获得的结果表明，橘子和甜橙汁中的夹杂物含量显着（p  <0.05）与未稀释精液相比，可增强进行性精子活力和精子动力学。稀释剂的抗氧化剂活性在0 h随果汁的加入呈比例增加，在5 h时，基于果汁的稀释剂的40％，50％和60％的抗氧化剂活性高于未稀释的精液。含果汁的稀释剂中脂质过氧化减少，未稀释精液和0％果汁中脂质过氧化率增加。总之，橘子和甜橙汁可抑制公鸡精液中脂质的过氧化，并增强精子的进行性，并维持公鸡精液的动力学。