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Oxidative status and spermatozoa kinetics of rooster semen in citrus juice-based diluent

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Abstract

Poultry spermatozoa are prone to oxidative damage and adversely affect their fertility. Bioactive constituents of citrus fruit confer antioxidant enrichment on its juice and are candidates to combat oxidative load in poultry semen. Computer-assisted semen analyser and oxidative status were used to evaluate the potency of two tropical citrus varieties (sweet orange and tangerine) as natural diluents for rooster semen. Fresh and ripe sweet orange and tangerine fruits were obtained and processed into juices using a standard protocol and included in dextrose saline at 0%, 10%, 20%, 30%, 40%, 50% and 60% as semen diluent. Semen pool from 30 breeder roosters of 35–40 weeks of age was allotted randomly in triplicates to the different fruit juice-dextrose at 1:2 dilution rate and evaluated for 5 h at room temperature. Diluted semen, according to treatments, was evaluated for sperm kinetics using a computer-assisted sperm analyser, and seminal plasma was assayed for lipid peroxidation and total antioxidant activity. The result obtained shows that tangerine and sweet orange juice inclusion significantly (p < 0.05) enhance progressive spermatozoa motility and semen kinetics compared favourably with undiluted semen. The diluents had a proportionate increase in antioxidant activity with juice inclusion at 0 h and the antioxidant activity of 40%, 50% and 60% fruit juice-based diluents was higher than undiluted semen at 5 h. There was a reduced lipid peroxidation in juice inclusive diluents and increase lipid peroxidation rate in undiluted semen and 0% juice inclusion. In conclusion, tangerine and sweet orange juice inhibit lipid peroxidation in rooster semen and enhance progressive spermatozoa motility and maintain rooster semen kinetics.

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Abbreviations

PUFAs:

polyunsaturated fatty acids

FR:

free radical

ROS:

reactive oxygen species

SWJ:

sweet orange juice

TGJ:

tangerine juice

SOD:

superoxide dismutase

GPx:

glutathione peroxidase

CAT:

catalase

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Funding

The research outcome presented in this article was funded by the TETFUND 2017 institutional-based research intervention of the Nigerian government.

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Authors and Affiliations

  1. Animal Production Unit, Department of Agricultural Technology, Federal Polytechnic Ado-Ekiti, Ado-Ekiti, Ekiti State, Nigeria

    O. A. Jimoh, E. S. Ayedun, S. O. Ayodele, S. I. Omoniyi, A. A. Lawal, O. A. Ademola & B. J. Kolawole

  2. Department of Agricultural Science, Adeyemi College of Education, Ondo, Nigeria

    A. D. Oladepo

Authors
  1. O. A. Jimoh
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  2. E. S. Ayedun
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  3. S. O. Ayodele
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  4. S. I. Omoniyi
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  5. A. D. Oladepo
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  6. A. A. Lawal
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  7. O. A. Ademola
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  8. B. J. Kolawole
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Contributions

Jimoh O.A. designed the study, carried out the experimental protocol and wrote the first draft. Ayedun E.S. co-supervised the study and managed the semen collection. Ayodele S.O. and Omoniyi I.S. supervised animal handling, management of animals and data collection of fertility trial. Lawal A.A., Ademola O.A. and Kolawole B.J. carried out the fertility protocol and management of animals. Oladepo A.D. evaluated the experimental protocol, managed the data and statistical analysis. All authors read and approved the final manuscript.

Corresponding author

Correspondence to O. A. Jimoh.

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The study was approved by the institutional committee on the care and use of animals for the experiment and in accordance with the NIH guide for the care and use of laboratory animals.

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The authors declare that they have no conflict of interest.

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Jimoh, O.A., Ayedun, E.S., Ayodele, S.O. et al. Oxidative status and spermatozoa kinetics of rooster semen in citrus juice-based diluent. Trop Anim Health Prod 53, 31 (2021). https://doi.org/10.1007/s11250-020-02482-5

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  • DOI: https://doi.org/10.1007/s11250-020-02482-5

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