Clitoria ternatea (L.) is a medicinal leguminous plant and is cultivated to cater the need of herbal industries and asthetic purposes. The unavailability of steady molecular marker impedes the genetic improvement of C. ternatea. In the present study, transferability of 98 pairs of Cajanus spp. specific SSR primers were assessed among 14 genotypes of C. ternatea, varied for their flower color, floral architecture and bio-metabolite (taraxerol and delphinidin) content, and out of them 43 had successfully amplified the fragments. Among them, 36 pairs of primers showed 100% transferability, whereas rest seven varied from 42.86 to 92.85% transferability. The transferable 43 pairs of SSR primers generated 196 alleles across the 14 genotypes and the AMOVA analysis showed moderate genetic variation (55.1%) among the genotypes of C. ternatea, which was also reinforced by Nei’s genetic distance and gene identity estimates derived haplotype matrix. Similarly, both the principal coordinate analysis and dendrogram grouped these 14 genotypes of C. ternatea into two major clusters based on SSR allele distribution and frequency, and the clustering pattern is in accordance with petal color but in contrast to floral architecture. MCheza based outlier analysis revealed 16 alleles for balancing selection, which are putatively involved in the maintenance of genetic polymorphism in C. ternatea. Moreover, the estimates of molecular diversity and bio-metabolite content revealed the possible use of these genotypes in future breeding programme of this species.

Clitoria ternatea (L.) 是一种药用豆科植物，是为了满足草药工业和审美目的而种植的。稳定分子标记的缺乏阻碍了C. ternatea的遗传改良。在本研究中，98 对Cajanus spp 的可转移性。在C. ternatea 的14 种基因型中评估了特定的 SSR 引物，其花色、花结构和生物代谢物（蒲公英醇和飞燕草素）含量各不相同，其中 43 个已成功扩增片段。其中，36 对引物显示出 100% 的可转移性，而其余 7 对的可转移性从 42.86 到 92.85% 不等。可转移的 43 对 SSR 引物在 14 个基因型中产生了 196 个等位基因，AMOVA 分析显示C. ternatea基因型之间存在中等遗传变异 (55.1%) ，Nei 的遗传距离和基因同一性估计衍生的单倍型矩阵也加强了这一点。类似地，主坐标分析和树状图对C. ternatea 的这 14 个基因型进行了分组根据 SSR 等位基因分布和频率分为两个主要集群，集群模式与花瓣颜色一致，但与花卉结构相反。基于MChez的离群值分析揭示了 16 个用于平衡选择的等位基因，它们被认为与C. ternatea中遗传多态性的维持有关。此外，分子多样性和生物代谢物含量的估计揭示了这些基因型在该物种未来育种计划中的可能用途。