In this report, an environment friendly and cost effective green synthesis method of magnetite nanoparticles (Fe₃O₄ NPs) was introduced. Fe₃O₄ NPs were successfully synthesized by green route using Ocimum basilicum leaf extract. The structural investigation of Fe₃O₄ NPs using X-Ray diffraction technique revealed the cubic crystallinity with Fd3m space group, which was confirmed with an aid of Rietveld refinement. No secondary phases (such as Fe₂O₃ and FeO) were observed, which confirmed the high purity of synthesized Fe₃O₄ NPs. The characteristics peak at 3320 cm⁻¹ displayed the phenolic constituents of Ocimum basilicum leaf extract and peak at 665 cm⁻¹ illustrated the Fe–O bending, which confirmed the formation of Fe₃O₄ NPs. Ocimum basilicum leaf extract mediated Fe₃O₄ NPs possess spherical morphology with an average particle size ~ 15 nm as depicted by transmission electron microscopy (TEM). The magnetic potential of Ocimum basilicum leaf extract mediated Fe₃O₄ NPs was also carried out by using a vibration sample magnetometer (VSM), which revealed the superparamagnetic behavior of Fe₃O₄ NPs. Cytotoxicity of the as-synthesized Fe₃O₄ NPs nanoparticles was also examined on the human breast cancer cell line, which illustrated the efficacy of the synthesized nanoparticles with a low value of IC₅₀ (~ 17.75 µg/ml). The results displayed the potent anticancerous effect of Ocimum basilicum leaf extract capped Fe₃O₄ NPs, which made these nanoparticles suitable for a drug-loaded vehicle for the drug delivery system for biomedical applications.

在此报告中，介绍了一种环保且经济高效的磁铁矿纳米颗粒（Fe ₃ O ₄ NPs）绿色合成方法。用罗勒叶提取物通过绿色途径成功合成了Fe ₃ O ₄ NP 。用X射线衍射技术对Fe ₃ O ₄ NPs的结构研究表明，立方晶体具有Fd3m空间群，这在Rietveld精炼的帮助下得以证实。没有观察到次级相（例如Fe ₂ O ₃和FeO），这证实了合成的Fe ₃ O ₄的高纯度。NP。在3320 cm ^-1处的特征峰显示了罗勒叶提取物的酚类成分，在665 cm ^-1处的峰表明了Fe–O弯曲，这证实了Fe ₃ O ₄ NP的形成。罗勒叶提取物介导的Fe ₃ O ₄ NP具有球形形态，平均粒径约为15 nm，如透射电子显微镜（TEM）所示。罗勒叶提取物介导的Fe ₃ O ₄的磁势还使用振动样品磁力计（VSM）进行了NPs的研究，该样品揭示了Fe ₃ O ₄ NPs的超顺磁行为。还在人乳腺癌细胞系上检测了合成后的Fe ₃ O ₄ NPs纳米颗粒的细胞毒性，这说明了合成的纳米颗粒具有低IC ₅₀值（〜17.75 µg / ml）的功效。结果显示了罗勒叶提取物加盖的Fe ₃ O ₄ NPs的强效抗癌作用，这使这些纳米颗粒适合于载有生物医学药物输送系统的载药载体。