Balance control is accomplished by the anatomical link which provides the neural information for the coordination of skeletal muscles. However, there are few experimental proofs to directly show the neuroanatomical connection. Here, we examined the behavioral alterations by constructing an animal model with chemically induced unilateral labyrinthectomy (UL). In the experiment using rats (26 for UL, 14 for volume cavity, 355–498 g, male), the models were initially evaluated by the rota-rod (RR) test (21/26, 80.8%) and ocular displacement (23/26, 88.5%). The duration on the rolling rod decreased from 234.71 ± 64.25 s (4th trial before UL) to 11.81 ± 17.94 s (1st trial after UL). Also, the ocular skewed deviation (OSD) was observed in the model with left (5.79 ± 3.06°) and right lesion (3.74 ± 2.69°). Paw distance (PW) was separated as the front (FPW) and the hind side (HPW), and the relative changes of HPW (1.71 ± 1.20 cm) was larger than those of FPW (1.39 ± 1.06 cm), providing a statistical significance (p = 1.51 × 10⁻⁴, t test). Moreover, the results of the RR tests matched to those of the changing rates (18/21, 85.7%), and the changes (16/18, 88.9%) were dominantly observed in HPW (in FPW, 2/18, 11.1%). Current results indicated that the UL directly affected the changes in HPW more than those in FPW. In conclusion, the missing neural information from the peripheral vestibular system caused the abnormal posture in HPW, and the postural instability might reduce the performance during the voluntary movement shown in the RR test, identifying the relation between the walking imbalance and the unstable posture in PW.

平衡控制是通过为骨骼肌的协调提供神经信息的解剖学链接来完成的。然而，很少有实验证据可以直接显示神经解剖学的联系。在这里，我们通过构建具有化学诱导的单侧迷路切除术 (UL) 的动物模型来检查行为改变。在使用大鼠的实验中（UL 26 只，容积腔 14 只，355-498 g，雄性），模型最初通过旋转杆（RR）测试（21/26，80.8%）和眼球位移（23 /26, 88.5%)。滚动棒的持续时间从 234.71 ± 64.25 s（UL 之前的第 4 次试验）减少到 11.81 ± 17.94 s（UL 之后的第 1 次试验）。此外，在左侧（5.79 ± 3.06°）和右侧病变（3.74 ± 2.69°）的模型中观察到眼球倾斜偏差（OSD）。p  = 1.51 × 10 ^-4，t检验）。此外，RR 测试的结果与变化率 (18/21, 85.7%) 匹配，并且变化 (16/18, 88.9%) 主要在 HPW 中观察到 (在 FPW, 2/18, 11.1% ）。目前的结果表明，UL 对 HPW 变化的直接影响大于对 FPW 的影响。总之，来自外周前庭系统的神经信息缺失导致了 HPW 中的姿势异常，而姿势不稳定性可能会降低 RR 测试中显示的自主运动期间的表现，从而确定步行不平衡与 PW 中不稳定姿势之间的关系.