Giant goldenrod (Solidago gigantea Ait.) root extract was screened for bioactive compounds by high-performance thin-layer chromatography (HPTLC), coupled with effect-directed analysis including antibacterial (Bacillus subtilis F1276, B. subtilis subsp. spizizenii, Aliivibrio fischeri and Xanthomonas euvesicatoria), antifungal (Fusarium avenaceum) and enzyme inhibition (acetyl- and butyrylcholinesterases, α- and β-glucosidases and α-amylase) assays. Compounds of six multipotent zones (Sg1-Sg6) were characterized by HPTLC-heated electrospray ionization-high-resolution mass spectrometry (HRMS) and HPTLC-Direct Analysis in Real Time-HRMS. Apart from zone Sg3, containing three compounds, a single characteristic compound was detectable in each bioactive zone. The bioassay-guided isolation using preparative-scale flash chromatography and high-performance liquid chromatography provided eight compounds that were identified by NMR spectroscopy as clerodane diterpenes. All isolates possessed inhibiting activity against at least one of the tested microorganisms.

通过高效薄层色谱法（HPTLC）筛选巨型金毛菊（Solidago gigantea Ait。）根提取物中的生物活性化合物，并进行包括抗菌药（枯草芽孢杆菌F1276，枯草芽孢杆菌亚种spizizenii，Aliivibrio fischeri和Xanthomonas euvesicatoria），抗真菌药（Fusarium avenaceum））和酶抑制（乙酰基和丁酰胆碱酯酶，α-和β-葡萄糖苷酶和α-淀粉酶）检测方法。通过HPTLC加热的电喷雾电离高分辨质谱（HRMS）和HPTLC直接实时HRMS分析来表征六个多能区（Sg1-Sg6）的化合物。除了包含三种化合物的Sg3区域外，在每个生物活性区域中都可以检测到单个特征性化合物。使用制备级快速色谱和高效液相色谱的生物测定指导分离提供了八种化合物，这些化合物已通过NMR光谱法鉴定为双氯丁烷二萜。所有分离物均具有针对至少一种测试微生物的抑制活性。