Bioactive clerodane diterpenes of giant goldenrod (Solidago gigantea Ait.) root extract

https://doi.org/10.1016/j.chroma.2020.461727Get rights and content
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Highlights

  • First time HPTLC-Fusarium avenaceum bioassay using mycelium suspension

  • HPTLC-EDA combined with HPTLC-DART-HRMS and HPTLC-ESI-HRMS

  • Preparative-scale isolation of eight multipotent clerodane diterpenes

  • First cholinesterase, glucosidase, amylase and microbial inhibitory potential of compounds

Abstract

Giant goldenrod (Solidago gigantea Ait.) root extract was screened for bioactive compounds by high-performance thin-layer chromatography (HPTLC), coupled with effect-directed analysis including antibacterial (Bacillus subtilis F1276, B. subtilis subsp. spizizenii, Aliivibrio fischeri and Xanthomonas euvesicatoria), antifungal (Fusarium avenaceum) and enzyme inhibition (acetyl- and butyrylcholinesterases, α- and β-glucosidases and α-amylase) assays. Compounds of six multipotent zones (Sg1-Sg6) were characterized by HPTLC-heated electrospray ionization-high-resolution mass spectrometry (HRMS) and HPTLC-Direct Analysis in Real Time-HRMS. Apart from zone Sg3, containing three compounds, a single characteristic compound was detectable in each bioactive zone. The bioassay-guided isolation using preparative-scale flash chromatography and high-performance liquid chromatography provided eight compounds that were identified by NMR spectroscopy as clerodane diterpenes. All isolates possessed inhibiting activity against at least one of the tested microorganisms.

Keywords

High-performance thin-layer chromatography – effect directed analysis
High-performance thin-layer chromatography – high-resolution mass spectrometry
Fusarium avenaceum
Giant goldenrod (Solidago gigantea Ait.)
Clerodane diterpenes
Antibacterials and antifungals

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