Serum is a common supplement that is widely used to protect various cells and tissues from cryopreservation because it provides the necessary active components for cell growth and maintenance. In this study, we compared the effects of newborn calf serum (NCS) and fetal bovine serum (FBS) on the cryopreservation of mouse spermatogonial stem cells (SSCs). The isolated SSCs were cryopreserved in two groups: freezing medium that contained 10% DMSO (dimethyl sulfoxide) and 10% FBS in DMEM (Dulbecco’s Modified Eagle’s Medium) (group 1) and freezing medium that contained 10% DMSO and 10% NCS in DMEM (group 2). Real-time PCR was performed for stemness gene expression. The SSCs' viability was performed by trypan blue. We observed that the SSCs had increased viability in the NCS-freeze/thaw group (87.82%) compared to the FBS-freeze/thaw group (79.83%), but this increase was not statistically significant (P < 0.105). Promyelocytic leukemia zinc finger (Plzf) and Lin28 gene expression levels in the NCS-frozen/thawed SSCs were not significantly different compared to the FBS-frozen/thawed SSCs; however, Nanog gene expression increased considerably, and Dazl gene expression decreased significantly. The results in this study demonstrated that the presence of NCS in a solution of cryopreserved SSCs increased their viability after freeze/thawing and might promote the proliferation of cultivated SSCs in vitro by increasing the relative expression of Nanog.

血清是一种常见的补充剂，由于它为细胞生长和维持提供了必要的活性成分，因此被广泛用于保护各种细胞和组织免于冷冻保存。在这项研究中，我们比较了新生小牛血清（NCS）和胎牛血清（FBS）对小鼠精原干细胞（SSCs）冷冻保存的影响。将分离的SSC冷冻保存为两类：在DMEM中含有10％DMSO（二甲亚砜）和10％FBS的冷冻培养基（Dulbecco改良Eagle培养基）（第1组）；在DMEM中含有10％DMSO和10％NCS的冷冻培养基（第2组）。对干基因表达进行实时PCR。SSC的生存力由锥虫蓝进行。我们观察到，与FBS冷冻/解冻组（79.83％）相比，NCS冷冻/解冻组中的SSC活力有所提高（87.82％），但这种增加没有统计学意义（P <0.105）。早幼粒细胞白血病锌指（与FBS冻结/解冻的SSC相比，NCS冻结/解冻的SSC中的Plzf）和Lin28基因表达水平没有显着差异。然而，Nanog基因表达显着增加，而Dazl基因表达显着减少。这项研究的结果表明，冷冻保存的SSC溶液中NCS的存在会增加其冻融后的活力，并可能通过增加Nanog的相对表达来促进培养的SSC的体外增殖。