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A comparison of the effects of fetal bovine serum and newborn calf serum on cell growth and maintenance of cryopreserved mouse spermatogonial stem cells

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Abstract

Serum is a common supplement that is widely used to protect various cells and tissues from cryopreservation because it provides the necessary active components for cell growth and maintenance. In this study, we compared the effects of newborn calf serum (NCS) and fetal bovine serum (FBS) on the cryopreservation of mouse spermatogonial stem cells (SSCs). The isolated SSCs were cryopreserved in two groups: freezing medium that contained 10% DMSO (dimethyl sulfoxide) and 10% FBS in DMEM (Dulbecco’s Modified Eagle’s Medium) (group 1) and freezing medium that contained 10% DMSO and 10% NCS in DMEM (group 2). Real-time PCR was performed for stemness gene expression. The SSCs' viability was performed by trypan blue. We observed that the SSCs had increased viability in the NCS-freeze/thaw group (87.82%) compared to the FBS-freeze/thaw group (79.83%), but this increase was not statistically significant (P < 0.105). Promyelocytic leukemia zinc finger (Plzf) and Lin28 gene expression levels in the NCS-frozen/thawed SSCs were not significantly different compared to the FBS-frozen/thawed SSCs; however, Nanog gene expression increased considerably, and Dazl gene expression decreased significantly. The results in this study demonstrated that the presence of NCS in a solution of cryopreserved SSCs increased their viability after freeze/thawing and might promote the proliferation of cultivated SSCs in vitro by increasing the relative expression of Nanog.

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Acknowledgements

This study was confirmed by the Lorestan University of Medical Sciences. The authors would like to thanks the Lorestan University of Medical Sciences, Khorramabad, Iran The authors also thank the head and staff of the Razi Herbal Medicines Research Center of Lorestan Medical University.

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Authors and Affiliations

  1. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

    Afshin Pirnia & Arezou Moghadam

  2. Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Vahideh Assadollahi & Masoud Alasvand

  3. Medical Technology Research Center, Institute of Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mohammad Reza Gholami

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  1. Afshin Pirnia
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  2. Vahideh Assadollahi
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  4. Arezou Moghadam
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  5. Mohammad Reza Gholami
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Correspondence to Mohammad Reza Gholami.

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Pirnia, A., Assadollahi, V., Alasvand, M. et al. A comparison of the effects of fetal bovine serum and newborn calf serum on cell growth and maintenance of cryopreserved mouse spermatogonial stem cells. Mol Biol Rep 47, 9609–9614 (2020). https://doi.org/10.1007/s11033-020-06004-2

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  • DOI: https://doi.org/10.1007/s11033-020-06004-2

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