Some miRNAs, including the miR-302 cluster, are critical regulators of the stemness state of embryonic stem cells and cell fate patterning. In this study, we evaluated the activity of the miR-302 core promotor in mice and human pluripotent stem cells, somatic tissue derivatives, and generated transgenic mice expressing EGFP under a miR-302 promoter. The expression of EGFP under the control of the miR-302 promotor was examined in the cell lines and somatic tissues of transgenic mice, transgenic blastocysts, and embryonic stem cells derived from transgenic blastocysts. Our results showed that the miR-302 promoter is highly expressed in the mouse and human pluripotent cells, weakly expressed in the somatic tissue derivatives, is highly expressed in both blastocysts and the first passages of transgenic embryonic stem cells, and lowly expressed in the somatic tissues of transgenic mice. It can be concluded that different temporal and spatial gene expression patterns occur during the embryonic and adult stages of cells in mice.

一些 miRNA，包括miR-302簇，是胚胎干细胞干细胞状态和细胞命运模式的关键调节因子。在这项研究中，我们评估了miR-302核心启动子在小鼠和人类多能干细胞、体细胞组织衍生物中的活性，并生成了在miR-302启动子下表达 EGFP 的转基因小鼠。在miR-302启动子控制下的 EGFP 表达在转基因小鼠、转基因囊胚和源自转基因囊胚的胚胎干细胞的细胞系和体细胞组织中进行了检查。我们的结果表明miR-302启动子在小鼠和人多能细胞中高表达，在体组织衍生物中弱表达，在胚泡和转基因胚胎干细胞的第一代中高表达，在转基因小鼠的体细胞中低表达。可以得出结论，在小鼠细胞的胚胎和成体阶段发生了不同的时间和空间基因表达模式。