Abstract
Some miRNAs, including the miR-302 cluster, are critical regulators of the stemness state of embryonic stem cells and cell fate patterning. In this study, we evaluated the activity of the miR-302 core promotor in mice and human pluripotent stem cells, somatic tissue derivatives, and generated transgenic mice expressing EGFP under a miR-302 promoter. The expression of EGFP under the control of the miR-302 promotor was examined in the cell lines and somatic tissues of transgenic mice, transgenic blastocysts, and embryonic stem cells derived from transgenic blastocysts. Our results showed that the miR-302 promoter is highly expressed in the mouse and human pluripotent cells, weakly expressed in the somatic tissue derivatives, is highly expressed in both blastocysts and the first passages of transgenic embryonic stem cells, and lowly expressed in the somatic tissues of transgenic mice. It can be concluded that different temporal and spatial gene expression patterns occur during the embryonic and adult stages of cells in mice.
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Acknowledgments
We are grateful to Katherine Patrice Gill for editing and critical reading of the manuscript. The authors thank Ernst-Martin Füchtbauer and Annette Christine Füchtbauer for valuable scientifically and technically supporting of the project.
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This research was funded by the Iranian Council for Stem Cell Sciences and Technology grant number 7436.
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KR, FF, BK, and SJM provided the conceptual idea and designed the study. KR designed and made the plasmids and did the primary stem cell works. KR and FF made the transgenic mice. KR, SP, and MN genotyped and selected the transgenic pups, performed all molecular and IHC experiments, and downstream data acquisition and analysis. BK did the ImageJ data analysis and EGFP signals quantification. KR, SP, MN, BK, SJM, and FF contributed to the project by significant insights. KR and FF drafted the manuscript. All authors edited and approved the final version of the manuscript to be published.
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Editor: Tetsuji Okamoto
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Rahimi, K., Parsa, S., Nikzaban, M. et al. Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines. In Vitro Cell.Dev.Biol.-Animal 56, 896–905 (2020). https://doi.org/10.1007/s11626-020-00516-8
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DOI: https://doi.org/10.1007/s11626-020-00516-8