Spot blotch (SB) caused by the fungus Cochliobolus sativus, is an economically important disease on barley worldwide. Molecular analysis of SB resistance using quantitative trait locus (QTL) analysis can improve the efficiency of the breeding process. In the current work, and to accelerate development of SB-resistant barley cultivars, AFLP markers linked to SB resistance have been identified by using F2 recombinant inbred lines derived from a cross between the resistant barley cv. Banteng and the susceptible cv. WI2291. Single marker analysis (SMA) based on t-test revealed that out of 82 AFLP markers; five AFLP markers AAC × CTG (700 bp), ACT × CAT (600 bp), ACT × CAT (400 bp), ACT × CTG (550 bp) and AGG × CAG (490 bp) were identified. The data showed that SMA-based t-test could be a useful analysis for dominant markers such AFLP in breeding programs, and that the AFLP detected markers can be employed in genomics-assisted breeding for selection of SB-resistant barley genotypes.

中文翻译：

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通过大麦（Hordeum vulgare L.）中的单一标记分析鉴定与抗斑点病性相关的 AFLP 标记

由真菌 Cochliobolus sativus 引起的斑点斑点病 (SB) 是世界范围内对大麦具有重要经济意义的疾病。使用数量性状基因座 (QTL) 分析对 SB 抗性进行分子分析可以提高育种过程的效率。在目前的工作中，为了加速 SB 抗性大麦品种的开发，已通过使用源自抗性大麦品种之间杂交的 F2 重组自交系鉴定了与 SB 抗性相关联的 AFLP 标记。万腾和易感的cv。WI2291。基于 t 检验的单标记分析 (SMA) 显示 82 个 AFLP 标记中的 鉴定了五个AFLP标记AAC×CTG（700bp）、ACT×CAT（600bp）、ACT×CAT（400bp）、ACT×CTG（550bp）和AGG×CAG（490bp）。