Skip to main content
SpringerLink
Log in

Identification of AFLP markers associated with spot blotch resistance through single marker analysis in barley (Hordeum vulgare L.)

  • Original Paper
  • Published:
Cereal Research Communications Aims and scope Submit manuscript

Abstract

Spot blotch (SB) caused by the fungus Cochliobolus sativus, is an economically important disease on barley worldwide. Molecular analysis of SB resistance using quantitative trait locus (QTL) analysis can improve the efficiency of the breeding process. In the current work, and to accelerate development of SB-resistant barley cultivars, AFLP markers linked to SB resistance have been identified by using F2 recombinant inbred lines derived from a cross between the resistant barley cv. Banteng and the susceptible cv. WI2291. Single marker analysis (SMA) based on t-test revealed that out of 82 AFLP markers; five AFLP markers AAC × CTG (700 bp), ACT × CAT (600 bp), ACT × CAT (400 bp), ACT × CTG (550 bp) and AGG × CAG (490 bp) were identified. The data showed that SMA-based t-test could be a useful analysis for dominant markers such AFLP in breeding programs, and that the AFLP detected markers can be employed in genomics-assisted breeding for selection of SB-resistant barley genotypes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Afanasenko OS, Koziakov AV, Hedlay PE, Lashina NM, Anisimova AV, Manninen O, Jalli M, Potokina EK (2015) Mapping of the loci controlling the resistance to Pyrenophora teres f. teres and Cochliobolus sativus in two double haploid barley populations. Rus J Genet 5:242–253

    Article  Google Scholar 

  • Arabi MIE (2005) Inheritance of partial resistance to spot blotch in barley. Plant Breeding 124:605–607

    Article  Google Scholar 

  • Arabi MIE, Jawhar M (2003) Pathotypes of Cochliobolus sativus (spot blotch) on barley in Syria. J Plant Pathol 85:193–196

    Google Scholar 

  • Arabi MIE, Jawhar M (2004) Identification of Cochliobolus sativus (spot blotch) isolates expressing differential virulence on barley genotypes in Syria. J Phytopathol 152:461–464

    Article  Google Scholar 

  • Arney DC (1951) Inheritance of resistance to spot blotch in barley seedling. Phytopathology 41:691–698

    Google Scholar 

  • Bilgic H, Steffenson BJ, Hayes PM (2006) Molecular mapping of loci conferring resistance to different pathotypes of the spot blotch pathogen in barley. Phytopathology 96:699–708

    Article  CAS  Google Scholar 

  • Clark RV (1979) Yield losses in barley cultivars caused by spot blotch. Can J Plant Pathol 1:113–117

    Article  CAS  Google Scholar 

  • Collard BCY, Jaufer MZZ, Brouwer JB, Pang ECK (2005) An introduction to markers, quantitative trait locus (QTL) mapping and marker-assisted selection for crop improvement: The basic concepts. Euphytica 142:169–196

    Article  CAS  Google Scholar 

  • Duveiller E, Sharma R (2009) Genetic improvement and crop management strategies to minimize yield losses in warm non-traditional wheat growing areas due to spot blotch pathogen Cochliobolus sativus. J Phytopathol 157:521–534

    Article  Google Scholar 

  • El-Demerdash ES, Elsherbeny EA, Salama YAM et al (2019) Genetic diversity analysis of some Egyptian Origanum and Thymus species using AFLP markers. J Genet Eng Biotechnol 17:13

    Article  Google Scholar 

  • Fetch TG, Steffenson BJ (1999) Rating scales for assessing infection responses of barley infected with Cochliobolus sativus. Plant Dis 83:213–217

    Article  Google Scholar 

  • Griffee F (1925) Correlated inheritance of botanical characters in barley and the manner of reaction to Helminthosporium sativum. J Agric Res 30:915–933

    Google Scholar 

  • Guo H, Yao Q, Chen L, Wang F, Lang X, Pang Y, Feng J, Zhou J, Lin R, Xu S (2019) Virulence and molecular diversity in the Cochliobolus sativus population causing barley spot blotch in China. Plant Dis 103:2252–2262

    Article  CAS  Google Scholar 

  • Kavita PSK, Yadav KJ, Dalbeer, (2017) In vitro evaluation of fungicides against Bipolaris sorokiniana causing spot blotch of barley (Hordeum vulgare L.). Int J Curr Microbiol App Sci 6:4734–4739

    Article  Google Scholar 

  • Knudsen I, Hockenhull J, Jensen DF (1995) Biocontrol of seedling diseases of barley and wheat caused by Fusarium culmorum and Bipolaris sorokiniana: effect of selected fungal antagonists on growth and yield components. Plant Pathol 44:467–477

    Article  Google Scholar 

  • Kuldeep T, Nandan R, Kumar U, Prasad LC, Chand R, Joshi AK (2008) Inheritance and identification of molecular markers associated with spot blotch (Cochliobolus sativus) resistance through microsatellites analysis in barley. Genet Mol Biol 31:734–742

    Article  CAS  Google Scholar 

  • Kumar J, Schafer P, Huckelhoven R, Langen G, Baltruschat H, Stein E, Nagarajan S, Kogel HK (2002) Bipolaris sorokiniana, a cereal pathogen of global concern: cytological and molecular approaches towards better control. Mol Plant Pathol 3:185–195

    Article  CAS  Google Scholar 

  • Kutcher HR, Bailey KL, Rossnagel BG, Legge WG (1994) Heritability of common root rot and spot blotch resistance in barley. Can J Plant Pathol 16:287–294

    Article  Google Scholar 

  • Mathre DE (1997) Compendium of barley diseases. American Phytopathological Society Press, St. Paul, MN, USA

    Google Scholar 

  • Prohens J, Anderson GJ, Blanca JM, Canizares J, Zuriaga E, Nuez F (2006) The implications of AFLP data for the systematics of the wild species of Solanum section Basarthrum. Syst Bot 31:208–216

    Article  Google Scholar 

  • Rehman S, Gyawali S, Amri A, Verma R PS (2020) First report of spot blotch of barley caused by Cochliobolus sativus in Morocco. Plant Dis ISSN: 0191–2917

  • Saghai-Maroof MA, Biyashev RM, Yang GP, Zang Q, Allard RW (1994) Extraordinarily polymorphic microsatellites DNA in barley species diversity, chromosomal locations, and population dynamics. Proc Natl Acad Sci USA 91:5466–6470

    Article  CAS  Google Scholar 

  • Sharma RC, Duveiller E (2006) Farmer participatory evaluation confirms higher grain yields in spring wheat using a selection index for spot blotch resistance, maturity and kernel weight. Euphytica 150:307–317

    Article  Google Scholar 

  • Singh S, Singh H, Sharma A, Meeta M, Singh B, Joshi N, Grover P, Al-Yassin A, Kumar S (2014) Inheritance of spot blotch resistance in barley (Hordeum vulgare L.). Can J Plant Sci 94:1203–1209

    Article  Google Scholar 

  • Steffenson BJ, Hayes PM, Kleinhofs A (1996) Genetics of seeding and adult plant resistance to net blotch (Pyrenophora teres f. teres) and spot blotch (Cochliobolus sativus) in barley. Theor Appl Genet 92:552–558

    Article  CAS  Google Scholar 

  • Steffenson BJ, Fetch TG, Jr, Wesenberg D, Bockelman H (1998) Sources of resistance to net and spot blotch in barley.(Abstr.) Phytopathology 88:S85

  • Van Leur JG, Ceccarrelli S, Grando S (1989) Diversity fordisease resistance in barley landraces from Syria and Jordan. Plant Breed 103:324–335

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23:4407–4414

    Article  CAS  Google Scholar 

  • Waugh R, Bonar N, Baird E, Thomas B, Graner A, Hayes P, Powell W (1997) Homology of AFLP products in three mapping populations of barley. Mol Gen Genet 255:311–321

    Article  CAS  Google Scholar 

  • Wilcoxson RD, Rasmusson DC, Miles MR (1990) Development of barley resistant to spot blotch and genetics of resistance. Plant Dis 74:207–210

    Article  Google Scholar 

  • Zadoks JC, Chang TT, Konzak CF (1974) A decimal code the growth stages of cereals. Weed Res 14:415–421

    Article  Google Scholar 

Download references

Acknowledgements

The authors would like to thank the Director General of AECS and the Head of Molecular biology and Biotechnology Department for their support this research.

Author information

Authors and Affiliations

  1. Department of Molecular Biology and Biotechnology, AECS, P. O. Box 6091, Damascus, Syria

    M. I. E. Arabi, A. AL. daoude, A. Shoaib & M. Jawhar

  2. Faculty of Science, Damascus University, P. O. Box 86, Damascus, Syria

    L. Mokrani

Authors
  1. M. I. E. Arabi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. AL. daoude
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Mokrani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Shoaib
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Jawhar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. I. E. Arabi.

Additional information

Communicated by A. Börner.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Arabi, M.I.E., daoude, A.A., Mokrani, L. et al. Identification of AFLP markers associated with spot blotch resistance through single marker analysis in barley (Hordeum vulgare L.). CEREAL RESEARCH COMMUNICATIONS 49, 285–290 (2021). https://doi.org/10.1007/s42976-020-00109-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s42976-020-00109-x

Keywords

Search

Navigation