For simultaneous analysis of four fat-soluble tocopherols (α-, β-, γ-, and δ-) in edible oils, an efficient and green method using deep eutectic solvent-based liquid-phase microextraction (DES-LPME) coupled with reversed-phase high-performance liquid chromatography (RP-HPLC) was developed. The DESs formed by different quaternary ammonium salts and ethanol were used as the extractants. Tetrabutylammonium chloride (TBAC)-ethanol DES at a molar ratio of 1:2 achieved the best extraction efficiency. Under the optimized conditions, the detection limits were in the range of 2.1–3.0 ng mL⁻¹. The intra-day and inter-day repeatability were in the ranges of 3.9–5.3% and 4.8–7.1%, respectively, and the recoveries for the real samples varied from 80.7% to 105.4%. The developed method was successfully employed for the determination of all four tocopherol homologues with an RP-HPLC system containing a COSMOSIL π-NAP column in five edible oils collected locally.

为了同时分析食用油中的四种脂溶性生育酚（α-、β-、γ- 和 δ-），这是一种使用深共晶溶剂型液相微萃取 (DES-LPME) 与反相结合的高效绿色方法开发了-相高效液相色谱（RP-HPLC）。由不同季铵盐和乙醇形成的DESs用作萃取剂。摩尔比为 1:2 的四丁基氯化铵 (TBAC)-乙醇 DES 获得最佳提取效率。在优化条件下，检测限在 2.1–3.0 ng mL ^-1范围内. 日内和日间重复性分别在 3.9-5.3% 和 4.8-7.1% 的范围内，实际样品的回收率从 80.7% 到 105.4% 不等。所开发的方法已成功用于使用包含 COSMOSIL π-NAP 色谱柱的 RP-HPLC 系统在当地收集的五种食用油中测定所有四种生育酚同系物。