Frailty is a risk factor for poor health outcomes in older adults. The aim of this study was to identify plasma proteomic biomarkers of frailty in 752 men and women older than 65 years of age from the InCHIANTI study. One thousand three hundred one plasma proteins were measured using an aptamer-based assay. Associations of each protein with frailty status were assessed using logistic regression and four proteins creatine kinase M-type (CKM), B-type (CKB), C-X-C motif chemokine ligand 13 (CXCL13), and thrombospondin 2 (THBS2) were associated with frailty status. Two proteins, cyclin-dependent kinase 5 (CDK5/CDK5R1) and interleukin 1 alpha (IL1A), were associated with worsening of frailty status over time in volunteers free of frailty at baseline. Using partial least squares discriminant analysis (PLS-DA), data of 1301 proteins was able to discriminate between frail and non-frail with a 2% error rate. The proteins with greater discriminatory ability represented the inflammation, blood coagulation, and cell growth pathways. The utility of these proteins as biomarkers of frailty should be further explored.

虚弱是老年人健康状况不佳的一个危险因素。本研究的目的是确定 InCHIANTI 研究中 752 名 65 岁以上男性和女性虚弱的血浆蛋白质组生物标志物。使用基于适体的测定法测量了 1,301 个血浆蛋白。使用逻辑回归评估每种蛋白质与虚弱状态的关联，四种蛋白质肌酸激酶 M 型 (CKM)、B 型 (CKB)、CXC 基序趋化因子配体 13 (CXCL13) 和血小板反应蛋白 2 (THBS2) 与虚弱相关地位。细胞周期蛋白依赖性激酶 5 (CDK5/CDK5R1) 和白细胞介素 1 α (IL1A) 这两种蛋白质与基线时没有虚弱的志愿者随着时间的推移虚弱状态恶化有关。使用偏最小二乘判别分析 (PLS-DA)，1301 种蛋白质的数据能够以 2% 的错误率区分脆弱和非脆弱。具有较强辨别能力的蛋白质代表炎症、凝血和细胞生长途径。应进一步探索这些蛋白质作为虚弱生物标志物的效用。