Omega-3 fatty acids sourced from microalgae are in demand due to their therapeutic potential and sustainability of the source. The present study was focused towards enhancing eicosapentaenoic acid (EPA) productivity in the marine Chrysophyte Monodopsis subterranea through media supplementation using the plant growth regulators gibberellic acid, methyl jasmonate and myoinositol. The effect of three concentrations (1 μM, 10 μM and 100 μM) of supplements on biomass yield, fatty acid composition, total fatty acid (TFA) and EPA productivity were analyzed at three phases of microalgal growth. Supplementation with 100 μM GA increased biomass yield and TFA productivity by 3.3-fold and 3.9-fold respectively at the mid-exponential phase and EPA productivity increased up to 3.2-fold at the late-exponential phase. The addition of 1 μM MeJA stimulated biomass accumulation to 2.7-fold at the late-stationary phase, but this effect was reduced to 1.1–1.4-fold at higher concentrations. The addition of 10 μM MI also showed a positive effect on TFA productivity and thus increased EPA productivity by 1.8-fold at the late-stationary phase. Supplementation with 100 μM GA, 100 μM MeJA and 10 μM MI at the late-stationary phase increased all fatty acids except 18:0 and 18:2. EPA was negatively correlated with 18:2 at all three growth phases. Principal components analysis indicated a strong relationship between key fatty acids and EPA at all three growth stages and associated with 100 μM GA.

由于其治疗潜力和来源的可持续性，因此需要来自微藻的Omega-3脂肪酸。本研究的重点是提高海洋金眼藻Monopodpsis地下的二十碳五烯酸（EPA）的生产率。通过使用植物生长调节剂赤霉素，茉莉酸甲酯和肌醇进行培养基补充。在微藻生长的三个阶段分析了三种浓度（1μM，10μM和100μM）的补充剂对生物量产量，脂肪酸组成，总脂肪酸（TFA）和EPA生产力的影响。在指数中期，补充100μMGA可使生物量产量和TFA生产率分别提高3.3倍和3.9倍，而在指数后期，EPA生产率提高至3.2倍。在静止后期，加入1μMMeJA刺激生物量积累至2.7倍，但在较高浓度下，这种作用降低至1.1-1.4倍。添加10μMMI也显示出对TFA生产率的积极影响，因此使EPA生产率提高了1。平稳后期为8倍。在稳定期后期补充100μMGA，100μMMeJA和10μMMI可以增加除18：0和18：2之外的所有脂肪酸。在所有三个生长期，EPA与18：2呈​​负相关。主成分分析表明，在所有三个生长阶段，关键脂肪酸与EPA之间都具有很强的关系，并且与100μMGA有关。