A triploid was obtained from loquat (Eriobotrya japonica cv. ‘Ninghaibai’) (2n = 2x = 34) × bronze loquat (Eriobotrya deflexa, 2n = 2x = 34). The leaves of the triploid had no petioles, similar to the female parent, and its stipules were obvious and dark green, similar to those of the male parent. There were signals of male parent DNA on ten chromosomes of the triploid according to genomic in situ hybridization (GISH) when DNA of bronze loquat was used as probe; the diploid hybrid also had ten chromosomes with signals of male parent DNA. All five selected simple sequence repeats (SSR) primers amplified specific loci of both parents. In particular, primer CH04c06 amplified both specific loci of the female parent but only amplified one specific locus of the male parent. The results of GISH and SSR marker analysis indicated that the 2n gamete which formed the triploid was from first-division restitution or pre-meiotic doubling of the female parent cv. ‘Ninghaibai’. The allotriploid can be used to breed seedless loquat germplasm with different flowering times from loquat directly or to breed new germplasm containing a few genomic components from bronze loquat, such as monosomic alien addition lines, monosomic alien substitution lines, and introgression lines. This is the first report of allotriploid in the genus Eriobotrya and it will provide a basis for breeding new loquat germplasms by distant hybridization and provide an ideal material for breeding new loquat varieties with different flowering time or time to maturity.

中文翻译：

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从 Eriobotrya japonica (Thunb.) Lindl × E. deflexa (Hemsl.) Nakai 获得由雌性 2n 配子发育的异源三倍体杂种

从枇杷 (Eriobotrya japonica cv.'Ninghaibai') (2n = 2x = 34) × 青铜枇杷 (Eriobotrya deflexa, 2n = 2x = 34) 中获得三倍体。三倍体叶片无叶柄，与母本相似，托叶明显呈深绿色，与父本相似。以青铜枇杷的DNA为探针，基因组原位杂交(GISH)表明，三倍体的10条染色体上有父本DNA的信号；二倍体杂种也有十条染色体，带有父本 DNA 的信号。所有五个选定的简单序列重复 (SSR) 引物都扩增了父母双方的特定基因座。特别是，引物 CH04c06 扩增了母本的两个特定基因座，但仅扩增了父本的一个特定基因座。GISH和SSR标记分析结果表明，形成三倍体的2n配子来自母本cv.的第一分裂恢复或减数分裂前加倍。'宁海白'。异源三倍体可用于直接从枇杷选育不同开花期的无核枇杷种质，也可从青铜枇杷选育出含有少量基因组成分的新种质，如单体外加系、单体外来替代系、基因渗入系等。这是枇杷属异源三倍体的首次报道，将为通过远缘杂交培育新的枇杷种质提供依据，为培育不同开花期或成熟期的枇杷新品种提供理想的材料。