Abstract
A triploid was obtained from loquat (Eriobotrya japonica cv. ‘Ninghaibai’) (2n = 2x = 34) × bronze loquat (Eriobotrya deflexa, 2n = 2x = 34). The leaves of the triploid had no petioles, similar to the female parent, and its stipules were obvious and dark green, similar to those of the male parent. There were signals of male parent DNA on ten chromosomes of the triploid according to genomic in situ hybridization (GISH) when DNA of bronze loquat was used as probe; the diploid hybrid also had ten chromosomes with signals of male parent DNA. All five selected simple sequence repeats (SSR) primers amplified specific loci of both parents. In particular, primer CH04c06 amplified both specific loci of the female parent but only amplified one specific locus of the male parent. The results of GISH and SSR marker analysis indicated that the 2n gamete which formed the triploid was from first-division restitution or pre-meiotic doubling of the female parent cv. ‘Ninghaibai’. The allotriploid can be used to breed seedless loquat germplasm with different flowering times from loquat directly or to breed new germplasm containing a few genomic components from bronze loquat, such as monosomic alien addition lines, monosomic alien substitution lines, and introgression lines. This is the first report of allotriploid in the genus Eriobotrya and it will provide a basis for breeding new loquat germplasms by distant hybridization and provide an ideal material for breeding new loquat varieties with different flowering time or time to maturity.
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Data availability
The datasets supporting the conclusions and a description of the complete protocols are included within the article.
Abbreviations
- AFLP:
-
Amplified fragment length polymorphism
- FDR:
-
First-division restitution
- GISH:
-
Genomic in situ hybridization
- PMD:
-
Post-meiotic genome doubling
- PRD:
-
Pre-meiotic doubling
- RAPD:
-
Random amplified polymorphic DNA
- SDR:
-
Second-division restitution
- SSR:
-
Simple-sequence repeat
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Acknowledgements
We thank our colleagues at the College of Horticulture and Landscape Architecture of Southwest University for their technical assistance.
Funding
This study was funded by the National Key R&D Program of China (No. 2019YFD1000905), Fundamental Research Funds for the Central Universities (XDJK2019AA001 & XDJK2020B058), Innovation Research Group Funds for Chongqing Universities (CXQT19005), and the Open Fund of the Key Laboratory of Loquat Germplasm Innovation and Utilization (Putian University), Fujian Province University (2019004).
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All authors contributed to the study conception and design. YJS and JBD designed the experiments. YJS performed the experiments and drafted the manuscript. GW contributed to the simple sequence repeat analysis. JBD, GW, WWC, and QGG edited the manuscript. QH, XLL, SKL, QGG and GLL supervised the project; QGG and GLL provided laboratory facilities and project funding. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Song, Y., Dang, J., Chen, W. et al. An allotriploid hybrid developed from a female 2n gamete was obtained from Eriobotrya japonica (Thunb.) Lindl × E. deflexa (Hemsl.) Nakai. Euphytica 216, 192 (2020). https://doi.org/10.1007/s10681-020-02719-2
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DOI: https://doi.org/10.1007/s10681-020-02719-2