Spillover of honey bee viruses have posed a significant threat to pollination services, triggering substantial effort in determining the host range of the viruses as an attempt to understand the transmission dynamics. Previous studies have reported infection of honey bee viruses in ants, raising the concern of ants serving as a reservoir host. Most of these studies, however, are restricted to a single, local ant population. We assessed the status (geographical distribution/prevalence/viral replication) and phylogenetic relationships of honey bee viruses in ants across the Asia–Pacific region, using deformed wing virus (DWV) and two widespread invasive ants, Paratrechina longicornis and Anoplolepis gracilipes, as the study system. DWV was detected in both ant species, with differential geographical distribution patterns and prevenance levels between them. These metrics, however, are consistent across the geographical range of the same ant species. Active replication was only evident in P. longicornis. We also showed that ant-associated DWV is genetically similar to that isolated from Asian populations of honey bees, suggesting that local acquisition of DWV by the invasive ants may have been common at least in some of our sampled regions. Transmission efficiency of DWV to local arthropods mediated by ant, however, may vary across ant species.

中文翻译：

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两种广泛入侵的蚂蚁中的变形翼病毒：地理分布，患病率和系统发育

蜜蜂病毒的外溢对授粉服务构成了重大威胁，引发了巨大的努力来确定病毒的宿主范围，从而试图了解传播动态。先前的研究已经报道了蜜蜂病毒感染了蚂蚁，引起了人们对蚂蚁作为宿主宿主的关注。但是，大多数这些研究仅限于单个本地蚂蚁种群。我们使用变形翼状病毒（DWV）和两种广泛入侵的蚂蚁Paratrechina longicornis和Anoplolepis gracilipes评估了亚太地区蚂蚁中蜜蜂病毒的状况（地理分布/流行/病毒复制）和系统发育关系。，作为学习系统。在这两种蚂蚁物种中均检测到了DWV，它们之间的地理分布模式和来源水平不同。但是，这些度量在相同蚂蚁物种的地理范围内是一致的。主动复制仅在P中可见。longicornis。我们还表明，与蚂蚁相关的DWV在遗传学上与从亚洲蜜蜂中分离到的遗传相似，这表明至少在我们的某些采样区域中，入侵性蚂蚁对本地DWV的采集可能很普遍。但是，DWV对蚂蚁介导的本地节肢动物的传播效率可能因蚂蚁种类而异。