Liver organoids have recently been applied as models for liver disease and drug screening, especially when combined with liver-on-a-chip technologies. Compared to hepatocyte-like cells, primary hepatocytes have high functionality but cannot maintain their function when cultured in vitro. Mesenchymal stem cells (MSCs) enhance hepatocyte function and maintain hepatocyte metabolism when co-cultured with hepatocytes. MSCs can help induced pluripotent stem cells to generate an organoid structure via the MSC-based traction force triggered by extracellular matrix (ECM) proteins. In this study, primary hepatocytes were co-cultured with MSCs on a liver-derived ECM to generate liver organoids within a short duration.

中文翻译：

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创建大鼠肝细胞类器官作为药物测试的体外模型。

肝类器官最近被用作肝病和药物筛查的模型，尤其是与肝片技术结合使用时。与肝细胞样细胞相比，原代肝细胞具有较高的功能性，但在体外培养时不能维持其功能。与肝细胞共培养时，间充质干细胞（MSC）可增强肝细胞功能并维持肝细胞代谢。的MSC可以帮助诱导多能干细胞，以产生类器官结构 通过由细胞外基质（ECM）蛋白触发的基于MSC的牵引力。在这项研究中，原代肝细胞与MSC在肝脏衍生的ECM上共培养，以在短时间内产生肝脏类器官。