The crucial need for alternative oil sources due to increasing energy demand has motivated research to find alternatives resources. In the current study, different fungal groups were isolated from diverse Egyptian localities for oil production screening as well as sugarcane bagasse management. A total of 499 fungal isolates were recovered from soil and dung samples; one isolate was identified as Mortierella wolfii AH12 using morphological and molecular techniques and was demonstrated to produce high lipid concentrations. Taguchi design was used to obtain higher lipid productivity. Accordingly, dry biomass, total lipids, and lipid percentage were 3.81 g l⁻¹, 1.57 g l⁻¹, and 41.2%, respectively, under the optimized culture conditions: 40 g glucose, 5 g peptone, pH 5 at 30 0°C for 10 days. Non-pretreated sugarcane bagasse was not only utilized as an alternative carbon source, but it was also an effective source of nutrients for mold sprouting and lipid biosynthesis using solid-state fermentation (SSF) technique. Promising fatty acid profile was deduced, where palmitic (PA) and oleic acid (OA) were predominant. Moreover, unsaturated fatty acids (USFAs) as linoleic acid (LA), Gamma linolenic acid (GLA), Dihomo gamma linolenic acid (DGLA), arachidonic acid (ARA) were present in low quantities according to GC–MS analysis. The study offers a promising result that serves in the management of agricultural waste, converting it into lipids with a promising profile which could be considered as a precursor for biodiesel.

由于能源需求的增长，对替代石油资源的迫切需求促使了寻找替代能源的研究。在当前的研究中，从埃及不同地方分离出不同的真菌群，以进行石油生产筛选和甘蔗渣处理。从土壤和粪便样本中总共回收了499种真菌分离物；使用形态学和分子技术鉴定出一种分离物为狼毒莫尔氏菌AH12，并证明其产生高脂质浓度。Taguchi设计用于获得更高的脂质生产率。因此，干生物质，总脂质和脂质百分比分别为3.81克升^-1，1.57克L- ^-1在优化的培养条件下分别为40％的葡萄糖和41.2％的葡萄糖：40 g葡萄糖，5 g ,、 pH 0在30 0°C下放置10天。未经预处理的甘蔗渣不仅用作替代碳源，而且还是使用固态发酵（SSF）技术进行霉菌发芽和脂质生物合成的有效营养素来源。推断出有希望的脂肪酸谱，其中棕榈酸（PA）和油酸（OA）占优势。此外，根据GC-MS分析，不饱和脂肪酸（USFA）如亚油酸（LA），伽玛亚麻酸（GLA），二高纯伽玛亚麻酸（DGLA），花生四烯酸（ARA）的含量较低。这项研究提供了有前途的结果，可用于农业废物的管理，将其转化为具有良好前景的脂质，可被视为生物柴油的前体。