Thermophilic solid-state anaerobic digestion (SS-AD) of agricultural wastes, i.e., corn straw, cattle manure, and vegetable waste, was carried out in this study. The effects of temperature (40-60°C), initial solid content (ISC, 17.5-32.5%), and C/N ratio (15-32 : 1) on biogas production were evaluated using a Box-Behnken experimental design (BBD) combined with response surface methodology (RSM). The results showed that optimization of process parameters is important to promote the SS-AD performance. All the factors, including interactive terms (except the ISC), were significant in the quadratic model for biogas production with SS-AD. Among the three operation parameters, the C/N ratio had the largest effect on biogas production, followed by temperature, and a maximum biogas yield of 241.4 mL gVS^-1 could be achieved at 47.3°C, , and . After 20 d of SS-AD, the microbial community structure under different conditions was characterized by high-throughput sequencing, showing that Firmicutes, Bacteroidetes, Chloroflexi, Synergistetes, and Proteobacteria dominated the bacterial community, and that Firmicutes had a competitive advantage over Bacteroidetes at elevated temperatures. The biogas production values and relative abundance of OPB54 and Bacteroidia after 20 d of SS-AD can be fitted well using a quadratic model, implying that OPB54 and Bacteroidia play important roles in the methanogenic metabolism for agricultural waste thermophilic SS-AD.

中文翻译：

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玉米秸秆、牛粪和蔬菜废弃物的高温固态厌氧消化：温度、总固体含量和 C/N 比的影响


本研究对农业废弃物（即玉米秸秆、牛粪和蔬菜废弃物）进行了高温固态厌氧消化（SS-AD）。使用 Box-Behnken 实验设计 (BBD) 评估温度 (40-60°C)、初始固体含量 (ISC，17.5-32.5%) 和 C/N 比 (15-32 : 1) 对沼气产量的影响）与响应面法（RSM）相结合。结果表明，工艺参数的优化对于提升SS-AD性能具有重要意义。所有因素，包括交互项（ISC 除外），在 SS-AD 沼气生产的二次模型中都很重要。三个运行参数中，C/N比对沼气产量影响最大，其次是温度，在47.3℃时可实现最大沼气产量241.4 mL gVS ^-1 ， ，和 。 SS-AD 20 d后，通过高通量测序对不同条件下的微生物群落结构进行表征，结果显示，厚壁菌门、拟杆菌门、 Chloroflexi 、协同菌门和变形菌门在细菌群落中占主导地位，并且厚壁菌门在各方面比拟杆菌门具有竞争优势。温度升高。 SS-AD 20 d 后的沼气产量和OPB54和Bacteroidia的相对丰度可以使用二次模型很好地拟合，这表明OPB54和Bacteroidia在农业废弃物嗜热 SS-AD 的产甲烷代谢中发挥着重要作用。