Thermophilic solid-state anaerobic digestion (SS-AD) of agricultural wastes, i.e., corn straw, cattle manure, and vegetable waste, was carried out in this study. The effects of temperature (40-60°C), initial solid content (ISC, 17.5-32.5%), and C/N ratio (15-32 : 1) on biogas production were evaluated using a Box-Behnken experimental design (BBD) combined with response surface methodology (RSM). The results showed that optimization of process parameters is important to promote the SS-AD performance. All the factors, including interactive terms (except the ISC), were significant in the quadratic model for biogas production with SS-AD. Among the three operation parameters, the C/N ratio had the largest effect on biogas production, followed by temperature, and a maximum biogas yield of 241.4 mL gVS^-1 could be achieved at 47.3°C, , and . After 20 d of SS-AD, the microbial community structure under different conditions was characterized by high-throughput sequencing, showing that Firmicutes, Bacteroidetes, Chloroflexi, Synergistetes, and Proteobacteria dominated the bacterial community, and that Firmicutes had a competitive advantage over Bacteroidetes at elevated temperatures. The biogas production values and relative abundance of OPB54 and Bacteroidia after 20 d of SS-AD can be fitted well using a quadratic model, implying that OPB54 and Bacteroidia play important roles in the methanogenic metabolism for agricultural waste thermophilic SS-AD.

中文翻译：

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玉米秸秆、牛粪和蔬菜废弃物的高温固态厌氧消化：温度、总固体含量和 C/N 比的影响

本研究对农业废弃物，即玉米秸秆、牛粪和蔬菜废弃物进行了高温固态厌氧消化 (SS-AD)。使用 Box-Behnken 实验设计 (BBD) 评估温度 (40-60°C)、初始固含量 (ISC, 17.5-32.5%) 和 C/N 比 (15-32:1) 对沼气生产的影响) 结合响应面方法 (RSM)。结果表明，工艺参数的优化对于提升 SS-AD 性能很重要。所有因素，包括交互项（ISC 除外），在 SS-AD 沼气生产的二次模型中都很重要。在三个操作参数中，C/N比对沼气产量的影响最大，其次是温度，最大沼气产量为241.4 mL gVS ^-1 可以在 47.3°C 下达到， ，和. 20 d SS-AD后，在不同条件下的微生物群落结构的特点是高通量测序，显示出厚壁菌门，拟杆菌，绿弯菌门， Synergistetes和变形菌占主导地位的细菌群落，而厚壁菌门有超过一个的竞争优势拟杆菌在升高的温度。使用二次模型可以很好地拟合 SS-AD 20天后 OPB54和拟杆菌属的沼气产量和相对丰度，这意味着OPB54和拟杆菌属 在农业废弃物嗜热 SS-AD 的产甲烷代谢中发挥重要作用。