Key message

Present study revealed that specific expression of TaYUC10.3 in wheat young seeds could increase the content of auxin, and protein.

Abstract

Auxin is a vital endogenous hormone in plants, which is involved in the regulation of various physiological and biochemical processes in plants. The flavin-containing monooxygenase encoded by the YUCCA gene is a rate-limiting enzyme in the tryptophan-dependent pathway of auxin synthesis. TaYUC10.3 was identified, cloned and found that it was abundantly expressed in wheat young seeds. In this study, a seed-specific expression vector of TaYUC10.3 was constructed with the promoter of 1Bx17 glutenin subunit gene and transformed wheat using the particle bombardment method. The quantitative RT-PCR showed that TaYUC10.3 was expressed in a large amount in young seeds of the transgenic lines. Plant hormone-targeted metabolomics showed that the auxin content of the transgenic lines was significantly increased compared with controls. The GC / MS non-targeted metabolite multiple statistical analyses showed that the variable importance in projection (VIP) of tryptophan reduced in the transgenic lines. Simultaneously, the VIP of indole acetic acid increased. The precursor amino acids for synthesizing some proteins and carbohydrates were upregulated in the transgenic lines. Subsequently, it was found that the protein content of the seeds of the transgenic TaYUC10.3 wheat was significantly higher than that of the control. The wet gluten content and sedimentation value of the transgenic TaYUC10.3 wheat were also high. This result indicated that TaYUC10.3 might participate in auxin synthesis and affects the protein content of wheat seeds.

中文翻译：

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TaYUC10的种子特异性表达显着增加小麦种子中的生长素和蛋白质含量

关键信息

目前的研究表明，TaYUC10.3在小麦幼苗中的特异性表达可以增加生长素和蛋白质的含量。

抽象的

生长素是植物体内一种重要的内源激素，参与植物体内各种生理生化过程的调控。YUCCA基因编码的含黄素单加氧酶是生长素合成的色氨酸依赖途径中的限速酶。TaYUC10.3被鉴定、克隆，发现它在小麦幼苗中大量表达。本研究以1Bx17麦谷蛋白亚基基因启动子构建TaYUC10.3种子特异性表达载体，并采用粒子轰击法转化小麦。定量 RT-PCR 显示TaYUC10.3在转基因株系的幼种子中大量表达。植物激素靶向代谢组学表明，与对照相比，转基因株系的生长素含量显着增加。GC/MS非靶向代谢物多重统计分析表明，转基因株系中色氨酸的预测变量重要性( VIP)降低。同时，吲哚乙酸的VIP增加。用于合成一些蛋白质和碳水化合物的前体氨基酸在转基因品系中被上调。随后发现转基因TaYUC10.3小麦种子的蛋白质含量明显高于对照。转基因TaYUC10.3的湿面筋含量和沉降值小麦也高。该结果表明TaYUC10.3可能参与生长素合成并影响小麦种子的蛋白质含量。