Background

Fragaria vesca, the woodland strawberry, is a diploid relative of the cultivated strawberry. A GA-deficient mutant was found in ethyl methanesulfonate (EMS)-mutagenized lines of the Fragaria vesca accession ‘Yellow Wonder’.

Objective

CYP714C2 was found to be differentially expressed using RNA-seq analysis. It is necessary to identify the function of this gene.

Methods

In order to identify the function of this gene, it was cloned and transformed into Arabidopsis thaliana.

Results

The DNA sequence of CYP714C2 was found to be 1940 bp in length, with an open reading frame (ORF) of 1539 bp that is predicted to encode a protein of 512 amino acids. The hydrophilicity of this protein is low and it is unstable. The highest relative expression of FvCYP714C2 was found in the leaves, followed by the pedicels, and low expression levels were found in the other tissues examined. Constitutive expression of FvCYP714C2 significantly promoted the growth of transgenic A. thaliana plants; transgenic Arabidopsis plants grew faster and grew well than wild type Col-0 plants. GA₁₊₃ contents of the genetically modified Arabidopsis lines were significantly higher than that in the wild type.

Conclusion

We conclude that FvCYP714C2 is a gene that functions in the gibberellin biosynthesis pathway in strawberry.

中文翻译：

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FvCYP714C2基因在林地草莓赤霉素合成中起重要作用

背景

Fragaria vesca是林地草莓，是栽培草莓的二倍体亲缘种。在草莓品种“黄色奇迹”的甲磺酸乙酯 (EMS) 诱变品系中发现了 GA 缺陷突变体。

客观的

使用RNA-seq分析发现CYP714C2差异表达。有必要确定该基因的功能。

方法

为了鉴定该基因的功能，将其克隆并转化到拟南芥中。

结果

发现CYP714C2的 DNA 序列长度为 1940 bp，开放阅读框 (ORF) 为 1539 bp，预计可编码 512 个氨基酸的蛋白质。这种蛋白质亲水性低，不稳定。FvCYP714C2的最高相对表达在叶子中发现，其次是花梗，在其他检查的组织中发现低表达水平。FvCYP714C2的组成型表达显着促进了转基因拟南芥植株的生长；转基因拟南芥植物比野生型 Col-0 植物生长得更快且生长良好。转基因拟南芥的GA ₁₊₃含量 品系明显高于野生型。

结论

我们得出结论，FvCYP714C2是一个在草莓赤霉素生物合成途径中起作用的基因。