Abstract
Background
Fragaria vesca, the woodland strawberry, is a diploid relative of the cultivated strawberry. A GA-deficient mutant was found in ethyl methanesulfonate (EMS)-mutagenized lines of the Fragaria vesca accession ‘Yellow Wonder’.
Objective
CYP714C2 was found to be differentially expressed using RNA-seq analysis. It is necessary to identify the function of this gene.
Methods
In order to identify the function of this gene, it was cloned and transformed into Arabidopsis thaliana.
Results
The DNA sequence of CYP714C2 was found to be 1940 bp in length, with an open reading frame (ORF) of 1539 bp that is predicted to encode a protein of 512 amino acids. The hydrophilicity of this protein is low and it is unstable. The highest relative expression of FvCYP714C2 was found in the leaves, followed by the pedicels, and low expression levels were found in the other tissues examined. Constitutive expression of FvCYP714C2 significantly promoted the growth of transgenic A. thaliana plants; transgenic Arabidopsis plants grew faster and grew well than wild type Col-0 plants. GA1+3 contents of the genetically modified Arabidopsis lines were significantly higher than that in the wild type.
Conclusion
We conclude that FvCYP714C2 is a gene that functions in the gibberellin biosynthesis pathway in strawberry.
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References
Cao F, Li H, Wang SM, Li XM, Dai HY, Zhang ZH (2017) Expression and functional analysis of FaPH01;H9 gene of strawberry (Fragaria × ananassa). J Integr Agric 16:580–590. https://doi.org/10.1016/S2095-3119(16)61433-8
Chang L, Zhang Z, Yang H, Li H, Dai HY (2007) Detection of strawberry RNA and DNA viruses by RT-PCR using total nucleic acid as a template. J Phytopathol 155:431–436. https://doi.org/10.1111/j.1439-0434.2007.01254.x
Clough SJ, Bent AF (1998) Floral dip: a simplified method for agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743. https://doi.org/10.1046/j.1365-313X.1998.00343.x
Duncan DB (1955) Multiple range and multiple F-tests. Biometrics 11:1–42. https://doi.org/10.2307/3001478
Ehlting J, Hamberger B, Million-Rousseau R, Werck-Reichhart D (2006) Cytochromes P450 in phenolic metabolism. Phytochem Rev 5:239–270
Kushiro T, Okamoto M, Nakabayashi K, Yamagishi K, Kitamura S, Asami T, Hirai N, Koshiba T, Kamiya Y, Nambara E (2004) The Arabidopsis cytochrome P450 CYP707A encodes ABA 8′-hydroxylases: key enzymes in ABA catabolism. EMBO J 23:1647–1656. https://doi.org/10.1038/sj.emboj.7600121
Li ZG, Hao YW, Yang YW, Deng W (2010) Molecular cloning and expression analysis of a cytochrome P450 gene in tomato. Plant Growth Regul 61:297–304
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods 25:402–408
Logacheva MD, Valiejo-Roman CM, Pimenov MG (2008) Confidence limits on phylogenies: an approach using the bootstrap. Plant Syst Evol 270:783–791. https://doi.org/10.1080/00837792.1905.10669550
Magome H, Nomura T, Hanada A, Takeda-Kamiya N, Ohnishi T, Shinma Y, Katsumata T, Kawaide H, Kamiya Y, Yamaguchi S (2013) CYP714B1 and CYP714B2 encode gibberellin 13-oxidases that reduce gibberellin activity in rice. Proc Natl Acad Sci USA 110:1947–1952. https://doi.org/10.1073/pnas.1215788110
Mizutani M (2012) Impacts of diversification of cytochrome P450 on plant metabolism. Biol Pharm Bull 35:824–832. https://doi.org/10.1248/bpb.35.824
Nei M, Kumar S (2000) Molecular Evolution and Phylogenetics. Oxford University Press, New York
Nelson D, Werck-Reichhart D (2011) A P450-centric view of plant evolution. Plant J 66:194–211. https://doi.org/10.1111/j.1365-313X.2011.04529.x
Nelson DR, Schuler MA, Paquette SM, Werck-Reichhart D, Bak S (2004) Comparative genomics of rice and Arabidopsis. Analysis of 727 cytochrome P450 genes and pseudogenes from a monocot and a dicot. Plant Physiol 135:756–772. https://doi.org/10.1104/pp.104.039826
Ohnishi T, Watanabe B, Sakata K, Mizutani M (2006) CYP724B2 and CYP90B3 function in the early C-22 hydroxylation steps of brassinosteroid biosynthetic pathway in tomato. Biosci Biotechnol Biochem 70:2071–2080. https://doi.org/10.1271/bbb.60034
Rho I, Hwang Y, Lee H, Lim B, Lee C (2012) Interspecific hybridization of diploids and octoploids in strawberry. Sci Hortic 134:46–52
Rzhetsky A, Nei M (1992) A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9:945–967. https://doi.org/10.1093/oxfordjournals.molbev.a040771
Saito S, Hirai N, Matsumoto C, Ohigashi H, Ohta D, Sakata K, Mizutani M (2004) Arabidopsis CYP707As encode (+)-abscisic acid 8′-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid. Plant Physiol 134:1439–1449. https://doi.org/10.1104/pp.103.037614
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Shulaev V, Sargent DJ, Crowhurst RN, Mockler TC, Folkerts O, Delcher AL et al (2011) The genome of woodland strawberry (Fragaria vesca). Nat Genet 43:109–116. https://doi.org/10.1038/ng.740
Takei K, Yamaya T, Sakakibara H (2004) Arabidopsis CYP735A1 and CYP735A2 encode cytokinin hydroxylases that catalyse the biosynthesis of trans-Zeatin. J Biol Chem 279:41866–41872. https://doi.org/10.1074/jbc.M406337200
Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 101:11030–11035. https://doi.org/10.1073/pnas.0404206101
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. https://doi.org/10.1093/molbev/mst197
Tanaka Y (2006) Flower colour and cytochromes P450. Phytochem Rev 5:283–291. https://doi.org/10.1007/s11101-006-9003-7
Wang SM, Li WJ, Liu YX, Li H, Ma Y, Zhang ZH (2017) Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq. J Integr Agric 16:828–844. https://doi.org/10.1016/S2095-3119(16)61448-X
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z (2011) Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation. Plant J 67:342–353. https://doi.org/10.1111/j.1365-313X.2011.04596.x
Zhu Y, Nomura T, Xu Y, Zhang Y, Peng Y, Mao B, Hanada A, Zhou H, Wang R, Li P, Zhu X, Mander LN, Kamiya Y, Yamaguchi S, He Z (2006) ELONGATED UPPERMOST INTERNODE encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice. Plant Cell 18:442–456. https://doi.org/10.1105/tpc.105.038455
Acknowledgements
This work was supported by Science and technology research project of Hubei Provincial Department of Education (B2019147)
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Guo, X., Xie, Z., Zhang, Y. et al. The FvCYP714C2 gene plays an important role in gibberellin synthesis in the woodland strawberry. Genes Genom 43, 11–16 (2021). https://doi.org/10.1007/s13258-020-01011-w
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DOI: https://doi.org/10.1007/s13258-020-01011-w