A new aerobic denitrifying bacterium ZYL was isolated from domestic wastewater sludge and identified as Acinetobacter haemolyticus (similarity 99%) by the 16S rDNA sequencing analysis. The strain could use nitrate, nitrite and ammonium as the sole N-source for growth with a final product of N2, demonstrating its good abilities for aerobic denitrification and heterotrophic nitrification. Single-factor experiment results showed that the effective removal of nitrate by strain ZYL occurred with carbon source sodium succinate, C/N 16-24, pH 5-9, temperature 20-40 °C, DO ≥ 4.84 mg/L. Ammonium was preferentially used by strain ZYL with nitrate and ammonium as the mixed nitrogen sources. According to nitrogen utilization, nitrogen balance analysis, enzyme assay and denitrifying gene amplification, nitrate was assimilated directly by the isolate for cell synthesis and also converted into N2 through aerobic denitrification. All these make strain ZYL an ideal choice for treating nitrogen-containing wastewater.

中文翻译：

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从生活废水中分离出的新型好氧反硝化菌 ZYL 的硝酸盐去除性能。

从生活污水污泥中分离出一株新型好氧反硝化菌ZYL，经16S rDNA测序鉴定为溶血不动杆菌（相似度99%）。该菌株可以使用硝酸盐、亚硝酸盐和铵作为生长的唯一氮源，最终产物为 N2，表明其良好的好氧反硝化和异养硝化能力。单因素实验结果表明，碳源琥珀酸钠，C/N 16-24，pH 5-9，温度20-40℃，DO≥4.84mg/L，菌株ZYL对硝酸盐的去除效果显着。ZYL菌株优先使用铵，硝酸盐和铵作为混合氮源。根据氮素利用、氮平衡分析、酶分析和反硝化基因扩增，硝酸盐被分离物直接同化用于细胞合成，并通过好氧反硝化作用转化为 N2。所有这些使 ZYL 菌株成为处理含氮废水的理想选择。