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Nitrate removal performances of a new aerobic denitrifier, Acinetobacter haemolyticus ZYL, isolated from domestic wastewater

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Abstract

A new aerobic denitrifying bacterium ZYL was isolated from domestic wastewater sludge and identified as Acinetobacter haemolyticus (similarity 99%) by the 16S rDNA sequencing analysis. The strain could use nitrate, nitrite and ammonium as the sole N-source for growth with a final product of N2, demonstrating its good abilities for aerobic denitrification and heterotrophic nitrification. Single-factor experiment results showed that the effective removal of nitrate by strain ZYL occurred with carbon source sodium succinate, C/N 16–24, pH 5–9, temperature 20–40 °C, DO ≥ 4.84 mg/L. Ammonium was preferentially used by strain ZYL with nitrate and ammonium as the mixed nitrogen sources. According to nitrogen utilization, nitrogen balance analysis, enzyme assay and denitrifying gene amplification, nitrate was assimilated directly by the isolate for cell synthesis and also converted into N2 through aerobic denitrification. All these make strain ZYL an ideal choice for treating nitrogen-containing wastewater.

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Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (Grant no. 51778397), the International Cooperation Projects of Shanxi Province (Grant no. 201603D421040), and the Key Research and Development Program of Shanxi Province (Grant no. 201903D311004).

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Authors and Affiliations

  1. Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, China

    Ying Wang, Yu-Lan Zou & Yong-Kang Lv

  2. Innovation Institute of Environmental Industry, College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, China

    Hu Chen & Yong-Kang Lv

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  1. Ying Wang
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  2. Yu-Lan Zou
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  3. Hu Chen
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Correspondence to Ying Wang or Hu Chen.

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Wang, Y., Zou, YL., Chen, H. et al. Nitrate removal performances of a new aerobic denitrifier, Acinetobacter haemolyticus ZYL, isolated from domestic wastewater. Bioprocess Biosyst Eng 44, 391–401 (2021). https://doi.org/10.1007/s00449-020-02451-0

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