The Wumeng semi-fine wool sheep is vital to the production system in Southwest China. To study the effects of nano-selenium (Nano-Se) poisoning in the Wumeng semi-fine wool sheep, poisoning model was established. A total of 20 animals with an average initial body weight (BW) of 35.57 ± 3.31 kg were used in this study. After 1-week-long acclimatization period, all animals were randomly divided into two groups: (1) control, with no any treatment; (2) Nano-Se group, orally administered 5 mg Nano-Se (BW/kg/day). There were 10 replications/group and one sheep/replication. The experiment lasted for 30 days. Compared with the control, hemoglobin, erythrocyte count, and packed cell volume in Nano-Se group markedly decreased (P < 0.01), and the activities of serum lactate dehydrogenase, glutamic oxaloacetic transaminase, cereal third transaminase, and alkaline phosphatase in Nano-Se group significantly increased (P < 0.01). T3 and FT3 in serum in Nano-Se group were greatly lower than those in the control (P < 0.01). Contents of T4, FT4, and TSH in serum in Nano-Se group were extremely higher than those in the control (P < 0.01). Levels of IgG, IgM, IgA, interleukin 2 (IL-2), and tumor necrosis factor-alpha (TNF-α) in Nano-Se group were much lower than those of the control (P < 0.01), but levels of interleukin 6 (IL-6) and interleukin-1β (IL-1β) were greatly higher than those in the control (P < 0.01). Compared with the control, serum superoxide dismutase, glutathione peroxide, total antioxidant capacity, and catalase from Nano-Se group extremely decreased (P < 0.01), and content of serum malondialdehyde in Nano-Se group markedly increased (P < 0.01). The current results indicated that the blood parameters in the Wumeng semi-fine wool sheep were affected by Nano-Se poisoning, and the immune function and antioxidant capacity were greatly reduced too.

中文翻译：

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纳米硒中毒对乌蒙半细毛羊免疫功能的影响。

乌蒙半细毛羊对西南地区的生产体系​​至关重要。为研究纳米硒（Nano-Se）中毒对乌蒙半细毛羊的影响，建立中毒模型。本研究共使用了 20 只平均初始体重 (BW) 为 35.57 ± 3.31 kg 的动物。经过1周的适应期后，将所有动物随机分为两组：（1）对照组，不做任何处理；(2)纳米硒组，口服5毫克纳米硒(BW/kg/day)。有 10 个重复/组和 1 只绵羊/重复。实验持续了30天。与对照组相比，纳米硒组血红蛋白、红细胞计数和细胞压积显着降低（P < 0.01），血清乳酸脱氢酶、谷草酰乙酸转氨酶、谷类第三转氨酶、Nano-Se组碱性磷酸酶和碱性磷酸酶显着升高（P < 0.01）。Nano-Se组血清中T3和FT3明显低于对照组（P < 0.01）。Nano-Se组血清中T4、FT4、TSH的含量显着高于对照组（P < 0.01）。Nano-Se组IgG、IgM、IgA、白细胞介素2（IL-2）和肿瘤坏死因子-α（TNF-α）水平远低于对照组（P < 0.01），但白细胞介素水平显着低于对照组（P < 0.01）。 6（IL-6）和白细胞介素1β（IL-1β）显着高于对照组（P <0.01）。与对照组相比，纳米硒组血清超氧化物歧化酶、过氧化谷胱甘肽、总抗氧化能力和过氧化氢酶显着降低（P < 0.01），而纳米硒组血清丙二醛含量显着升高（P < 0.01）。