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Effects of Nano-selenium Poisoning on Immune Function in the Wumeng Semi-fine Wool Sheep

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Abstract

The Wumeng semi-fine wool sheep is vital to the production system in Southwest China. To study the effects of nano-selenium (Nano-Se) poisoning in the Wumeng semi-fine wool sheep, poisoning model was established. A total of 20 animals with an average initial body weight (BW) of 35.57 ± 3.31 kg were used in this study. After 1-week-long acclimatization period, all animals were randomly divided into two groups: (1) control, with no any treatment; (2) Nano-Se group, orally administered 5 mg Nano-Se (BW/kg/day). There were 10 replications/group and one sheep/replication. The experiment lasted for 30 days. Compared with the control, hemoglobin, erythrocyte count, and packed cell volume in Nano-Se group markedly decreased (P < 0.01), and the activities of serum lactate dehydrogenase, glutamic oxaloacetic transaminase, cereal third transaminase, and alkaline phosphatase in Nano-Se group significantly increased (P < 0.01). T3 and FT3 in serum in Nano-Se group were greatly lower than those in the control (P < 0.01). Contents of T4, FT4, and TSH in serum in Nano-Se group were extremely higher than those in the control (P < 0.01). Levels of IgG, IgM, IgA, interleukin 2 (IL-2), and tumor necrosis factor-alpha (TNF-α) in Nano-Se group were much lower than those of the control (P < 0.01), but levels of interleukin 6 (IL-6) and interleukin-1β (IL-1β) were greatly higher than those in the control (P < 0.01). Compared with the control, serum superoxide dismutase, glutathione peroxide, total antioxidant capacity, and catalase from Nano-Se group extremely decreased (P < 0.01), and content of serum malondialdehyde in Nano-Se group markedly increased (P < 0.01). The current results indicated that the blood parameters in the Wumeng semi-fine wool sheep were affected by Nano-Se poisoning, and the immune function and antioxidant capacity were greatly reduced too.

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Funding

This work was financially supported by the Project of National Key Research and Development Program of China in 13th Five-year Plan (2016YFC0502601) and the National Natural Science Foundation of China (41671041).

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Authors and Affiliations

  1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China

    Yuanfeng Li, Jian He & Xiaoyun Shen

  2. State Engineering Technology Institute for Karst Desertification Control, Guizhou Normal University, Guiyang, 550025, China

    Xiaoyun Shen

  3. World Bank Poverty Alleviation Project Office in Guizhou, Southwest China, Guiyang, 550004, Guizhou, China

    Xiaoyun Shen

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  1. Yuanfeng Li
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Correspondence to Xiaoyun Shen.

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All animals were cared for as outlined in the Guide for the Care and Use of Animals in Research and Teaching Consortium. Sample collections from animals were approved by the Institute of Zoology, Chinese Academy of Sciences, Institutional Animal Care and Use Committee (Project A0169).

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Jian He equally contributes to this work and is a co-first author.

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Li, Y., He, J. & Shen, X. Effects of Nano-selenium Poisoning on Immune Function in the Wumeng Semi-fine Wool Sheep. Biol Trace Elem Res 199, 2919–2924 (2021). https://doi.org/10.1007/s12011-020-02408-0

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