A 30-day feeding trial was conducted to investigate the effect of κ-selenocarrageenan on the growth performance, selenium accumulation, antioxidant capacity, and intestinal microbiota of sea cucumbers Apostichopus japonicus, with different sizes (70 g ± 10 g and 100 g ± 10 g). Sea cucumbers of each size were randomly assigned into two groups; a diet without supplemented κ-selenocarrageenan was referred to as a control diet, or supplemented with κ-selenocarrageenan at selenium (Se) levels of 2.0 μg/g. Selenium accumulation in the body wall and intestine was determined on days 0, 10, 20, and 30. The survival rate (SR) was significantly higher in the κ-selenocarrageenan-treated group (Se group) than in the control group. After 30 days of feeding, κ-selenocarrageenan supplementation increased the activities of glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC), and decreased malondialdehyde (MDA) levels in A. japonicus. Furthermore, the intestinal microbiota diversity of sea cucumbers was increased by dietary supplementation with κ-selenocarrageenan and the relative abundances of some probiotics (such as Sulfitobacter and Rhodobacteraceae) were also increased. It is suggested that κ-selenocarrageenan could increase the antioxidant capacity and modulate the intestinal microbiota of sea cucumbers A. japonicus. Further researches will be conducted for its optimal administration concentrations in vivo.

中文翻译：

---

膳食补充剂 κ-硒代角叉菜胶对海参 Apostichopus japonicus 硒积累和肠道微生物群的影响。

一项为期 30 天的饲养试验，旨在研究 κ-硒代角叉菜胶对不同规格（70 g ± 10 g 和 100 g ± 10 G）。将各种规格的海参随机分为两组；没有补充 κ-硒代角叉菜胶的饮食被称为对照饮食，或补充有 2.0 μg/g 硒 (Se) 水平的 κ-硒代角叉菜胶。在第 0、10、20 和 30 天测定了体壁和肠道中硒的积累。κ-硒代角叉菜胶治疗组（Se 组）的存活率（SR）显着高于对照组。喂食30天后，补充 κ-硒代角叉菜胶会增加谷胱甘肽过氧化物酶 (GSH-Px) 的活性和总抗氧化能力 (T-AOC)，并降低 A. japonicus 中的丙二醛 (MDA) 水平。此外，通过膳食补充 κ-硒代角叉菜胶增加了海参肠道微生物群的多样性，并且一些益生菌（如硫化杆菌和红杆菌科）的相对丰度也增加了。表明κ-硒代角叉菜胶可以提高海参的抗氧化能力并调节海参的肠道微生物群。将对其在体内的最佳给药浓度进行进一步研究。通过膳食补充 κ-硒代角叉菜胶增加了海参肠道微生物群的多样性，并且一些益生菌（如硫化杆菌和红杆菌科）的相对丰度也增加了。表明κ-硒代角叉菜胶可以提高海参的抗氧化能力并调节海参的肠道微生物群。将对其在体内的最佳给药浓度进行进一步研究。通过膳食补充 κ-硒代角叉菜胶增加了海参肠道微生物群的多样性，并且一些益生菌（如硫化杆菌和红杆菌科）的相对丰度也增加了。表明κ-硒代角叉菜胶可以提高海参的抗氧化能力并调节海参的肠道微生物群。将对其在体内的最佳给药浓度进行进一步研究。